Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
This affinity-purified antibody is directed against the phosphorylated form of Human Smad3 protein at the pSer423 and pSer425 residues. The product was affinity purified from monospecific antiserum by Immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against Human Smad3 pSer423 and pSer425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members.
Aufreinigung
Immunoaffinity Chromatography.
Immunogen
Synthetic peptide corresponding to amino acids 417-425 of Human SMAD3 protein
Suitable for use in ELISA, Immunohistochemistry and Western blot. Expect a bandapproximately 48 kDa in size corresponding to phosphorylated Smad3 protein by Westernblotting in the appropriate stimulated tissue or cell lysate or extract. Less than 0.2%reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested. Recommended Dilutions: ELISA: 1/75,000-1/300,000. Western Blot: 1/2,000-1/20,000. Immunohistochemistry: 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
HSPC193 antikoerper, HsT17436 antikoerper, JV15-2 antikoerper, LDS1C antikoerper, LDS3 antikoerper, MADH3 antikoerper, madh3 antikoerper, madh3a antikoerper, smad3 antikoerper, wu:fa99e03 antikoerper, XSmad3 antikoerper, XenMLP antikoerper, Xmad3 antikoerper, madh3-A antikoerper, Madh3 antikoerper, AU022421 antikoerper, Smad 3 antikoerper, mad3 antikoerper, madh3b antikoerper, zgc:92234 antikoerper, SMAD family member 3 antikoerper, SMAD family member 3a antikoerper, SMAD family member 3 L homeolog antikoerper, SMAD family member 3b antikoerper, SMAD3 antikoerper, smad3a antikoerper, smad3.L antikoerper, Smad3 antikoerper, smad3b antikoerper
Hintergrund
Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-beta pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated RSmad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-beta- mediated response. Alternate names: JV15-2, MAD homolog 3, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, SMAD family member 3, SMAD-3