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SMAD3 Antikörper (SMAD, Mothers Against DPP Homolog 3) pSer423, pSer425 Primary Antibody

SMAD3 Reaktivität: Human IHC, ELISA, WB Wirt: Kaninchen Polyclonal unconjugated
Pubmed (18 references)
Produktnummer ABIN129675
$658.50
Zzgl. Versandkosten $45.00 und $20.00 Trockeneis
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  • Target Alle SMAD3 Antikörper anzeigen
    SMAD3
    Bindungsspezifität
    • 34
    • 33
    • 33
    • 28
    • 23
    • 16
    • 14
    • 12
    • 11
    • 11
    • 10
    • 10
    • 10
    • 7
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 417-425, pSer423, pSer425
    Reaktivität
    • 296
    • 182
    • 166
    • 33
    • 13
    • 13
    • 11
    • 11
    • 9
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 278
    • 31
    Kaninchen
    Klonalität
    • 268
    • 41
    Polyklonal
    Konjugat
    • 160
    • 23
    • 16
    • 14
    • 13
    • 10
    • 8
    • 8
    • 6
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    Dieser SMAD3 Antikörper ist unkonjugiert
    Applikation
    • 218
    • 143
    • 140
    • 84
    • 83
    • 70
    • 55
    • 53
    • 31
    • 30
    • 13
    • 10
    • 5
    • 5
    • 2
    • 1
    Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
    Kreuzreaktivität
    Xenopus laevis, Xenopus tropicalis, Zebrafisch (Brachydanio rerio), Ratte (Rattus), Maus, Schwein, Schaf, Huhn
    Produktmerkmale
    Concentration Definition: by UV absorbance at 280 nm
    Immunogen
    This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 417-425 of human SMAD3 protein.
    Isotyp
    IgG
  • Applikationshinweise
    This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.  Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
  • Hutchinson, Darling, Nicolaou, Gori, Squair, Cohen, Hill, Sapkota: "Salt-inducible kinases (SIKs) regulate TGFβ-mediated transcriptional and apoptotic responses." in: Cell death & disease, Vol. 11, Issue 1, pp. 49, (2020) (PubMed).

    Stappenbeck, Wang, Tang, Zhang, Parhami: "Inhibition of Non-Small Cell Lung Cancer Cells by Oxy210, an Oxysterol-Derivative that Antagonizes TGFβ and Hedgehog Signaling." in: Cells, Vol. 8, Issue 10, (2020) (PubMed).

    Feng, Tang, Huang, Sun, You, Xiao, Lv, Xu, Lan: "TGF-β Mediates Renal Fibrosis via the Smad3-Erbb4-IR Long Noncoding RNA Axis." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 26, Issue 1, pp. 148-161, (2019) (PubMed).

    Subramanian, Kanzaki, Galloway, Schilling: "Mechanical force regulates tendon extracellular matrix organization and tenocyte morphogenesis through TGFbeta signaling." in: eLife, Vol. 7, (2019) (PubMed).

    Gao, Kanasaki, Li, Kitada, Okazaki, Koya: "βklotho is essential for the anti-endothelial mesenchymal transition effects of N-acetyl-seryl-aspartyl-lysyl-proline." in: FEBS open bio, Vol. 9, Issue 5, pp. 1029-1038, (2019) (PubMed).

    Tang, Tang, Xu, Li, Deng, Zhang: "Generation of Smurf2 Conditional Knockout Mice." in: International journal of biological sciences, Vol. 14, Issue 5, pp. 542-548, (2018) (PubMed).

    Li, Chung, Li, Overstreet, Gagnon, Grouix, Leduc, Laurin, Zhang, Harris: "Fatty acid receptor modulator PBI-4050 inhibits kidney fibrosis and improves glycemic control." in: JCI insight, Vol. 3, Issue 10, (2018) (PubMed).

    Li, Shi, Srivastava, Kitada, Nagai, Nitta, Kohno, Kanasaki, Koya: "FGFR1 is critical for the anti-endothelial mesenchymal transition effect of N-acetyl-seryl-aspartyl-lysyl-proline via induction of the MAP4K4 pathway." in: Cell death & disease, Vol. 8, Issue 8, pp. e2965, (2018) (PubMed).

    Chung, Overstreet, Li, Wang, Niu, Wang, Fan, Sasaki, Jin, Khodo, Gewin, Zhang, Harris: "TGF-β promotes fibrosis after severe acute kidney injury by enhancing renal macrophage infiltration." in: JCI insight, Vol. 3, Issue 21, (2018) (PubMed).

    Tang, Heller, Meng, Yu, Tang, Zhou, Zhang: "Transforming Growth Factor-β (TGF-β) Directly Activates the JAK1-STAT3 Axis to Induce Hepatic Fibrosis in Coordination with the SMAD Pathway." in: The Journal of biological chemistry, Vol. 292, Issue 10, pp. 4302-4312, (2017) (PubMed).

    Subathra, Korrapati, Howell, Arthur, Shayman, Schnellmann, Siskind: "Kidney glycosphingolipids are elevated early in diabetic nephropathy and mediate hypertrophy of mesangial cells." in: American journal of physiology. Renal physiology, Vol. 309, Issue 3, pp. F204-15, (2015) (PubMed).

    Luo, Xu, Chen, Warburton, Dong, Qian, Selman, Gauldie, Kolb, Shi: "A novel profibrotic mechanism mediated by TGFβ-stimulated collagen prolyl hydroxylase expression in fibrotic lung mesenchymal cells." in: The Journal of pathology, Vol. 236, Issue 3, pp. 384-94, (2015) (PubMed).

    Herhaus, Al-Salihi, Macartney, Weidlich, Sapkota: "OTUB1 enhances TGFβ signalling by inhibiting the ubiquitylation and degradation of active SMAD2/3." in: Nature communications, Vol. 4, pp. 2519, (2014) (PubMed).

    Kawamura, Maeda, Imamura, Setoguchi, Yokouchi, Ishidou, Komiya: "SnoN suppresses maturation of chondrocytes by mediating signal cross-talk between transforming growth factor-β and bone morphogenetic protein pathways." in: The Journal of biological chemistry, Vol. 287, Issue 34, pp. 29101-13, (2012) (PubMed).

    Beckham, Tuttle, Tyler: "Reovirus activates transforming growth factor beta and bone morphogenetic protein signaling pathways in the central nervous system that contribute to neuronal survival following infection." in: Journal of virology, Vol. 83, Issue 10, pp. 5035-45, (2009) (PubMed).

    Coffman, Coluccio, Planchart, Robertson: "Oral-aboral axis specification in the sea urchin embryo III. Role of mitochondrial redox signaling via H2O2." in: Developmental biology, Vol. 330, Issue 1, pp. 123-30, (2009) (PubMed).

    Yamashita, Fatyol, Jin, Wang, Liu, Zhang: "TRAF6 mediates Smad-independent activation of JNK and p38 by TGF-beta." in: Molecular cell, Vol. 31, Issue 6, pp. 918-24, (2008) (PubMed).

    Shi, Massagué: "Mechanisms of TGF-beta signaling from cell membrane to the nucleus." in: Cell, Vol. 113, Issue 6, pp. 685-700, (2003) (PubMed).

  • Target
    SMAD3
    Andere Bezeichnung
    SMAD3 (SMAD3 Produkte)
    Synonyme
    madh3, madh3a, smad3, wu:fa99e03, HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, XSmad3, XenMLP, Xmad3, madh3-A, Madh3, AU022421, Smad 3, mad3, SMAD family member 3, SMAD family member 3a, SMAD family member 3 L homeolog, SMAD3, smad3a, smad3.L, Smad3
    Hintergrund
    This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase.   These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily.  Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway.  Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes.  Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
    Synonyms: hMAD 3 antibody, hSMAD3 antibody, MADH3 antibody, MGC60396 antibody, Mothers against decapentaplegic homolog 3 antibody, Mothers against DPP homolog 3 antibody
    Gen-ID
    4088, 5174513
    UniProt
    P84022
    Pathways
    Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie
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