This affinity purified antibody is directed against DELTA-4. Expect a band ~74 kDa in size corresponding to DELTA-4 by Western blotting in the appropriate cell lysate or extract.
Kreuzreaktivität (Details)
Species reactivity (expected):Mouse, Rat and Chimpanzee (91 %). Species reactivity (tested):Human.
Aufreinigung
Immunoaffinity Chromatography
Immunogen
Synthetic peptide corresponding to an internal region of Human DELTA-4
DLL4
Reaktivität: Human
WB, ELISA, IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: 1/5,000-1/25,000. Western blot: 1/500-1/2,500. Immunohistochemistry on Paraffin Sections: 10-20 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
DELTA-4 (also called delta 4 precursor, delta ligand 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4 and notch ligand DLL4 precursor) is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. DELTA-4 plays a role in the Notch signaling pathway and activates Notch-1 and Notch-4. DELTA-4 is a membrane protein that is expressed in the vascular endothelium.Synonyms: Delta 4, Drosophila Delta homolog 4