GLI3 Antikörper (AA 41-57)

Produktnummer ABIN401281

Produktdetails anti-GLI3 Antikörper

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Bindungsspezifität: AA 41-57

Reaktivität: Human, Maus, Ratte, Hund, Huhn, Affe, Schimpanse, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLI3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody is directed against Gli-3 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Chimpanzee, squirrel Monkey, Xenopus laevis, Chicken, Canine (100 %).Mouse (94 %).Rat (88 %).
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 41-57 of Human Gli-3 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/6,000-1/30,000. Western blot: 1/500-1/2,000. Immunohistochemistry on Paraffin Sections: 0.5 mg/mL-5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M NaCl, pH 7.2, 0.09 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Andere Bezeichnung: GLI3 (GLI3 Produkte)

Synonyme: ACLS antikoerper, GCPS antikoerper, GLI3-190 antikoerper, GLI3FL antikoerper, PAP-A antikoerper, PAPA antikoerper, PAPA1 antikoerper, PAPB antikoerper, PHS antikoerper, PPDIV antikoerper, xgli3 antikoerper, AI854843 antikoerper, AU023367 antikoerper, Bph antikoerper, Pdn antikoerper, Xt antikoerper, add antikoerper, im:7142603 antikoerper, GLI3 antikoerper, GLI family zinc finger 3 antikoerper, GLI family zinc finger 3 L homeolog antikoerper, GLI-Kruppel family member GLI3 antikoerper, GLI3 antikoerper, Gli3 antikoerper, gli3.L antikoerper, gli3 antikoerper

Hintergrund: Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-polysyndactyly syndrome (GCPS), an autosomal dominant disorder-affecting limb and craniofacial development. Two isoforms of human Gli-3 have been reported. One is the fulllength protein at ~170-190 kDa and the other is a truncated isoform at ~80 kDa.Synonyms: GLI-3, Zinc finger protein GLI3

Gen-ID: 2737

NCBI Accession: NP_000159

UniProt: P10071

Pathways: Hedgehog Signalweg