ATF3 Antikörper (AA 113-130)

Produktnummer ABIN401262

Produktdetails anti-ATF3 Antikörper

Target: ATF3 (Activating Transcription Factor 3 (ATF3))

Bindungsspezifität: AA 113-130

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATF3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody is directed against ATF3 and is useful in determining its presence in various assays including ELISA and western blotting. It recognizes over-expressed ATF3 protein found in various expression systems.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5,000 to 1: 20,000. Western blot: 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Details zu ATF3

Target: ATF3 (Activating Transcription Factor 3 (ATF3))

Andere Bezeichnung: ATF3 (ATF3 Produkte)

Synonyme: zgc:55526 antikoerper, ATF3 antikoerper, LRG-21 antikoerper, LRF-1 antikoerper, LRFI antikoerper, atf-3 antikoerper, activating transcription factor 3 antikoerper, activating transcription factor 3 L homeolog antikoerper, atf3 antikoerper, ATF3 antikoerper, Atf3 antikoerper, atf3.L antikoerper

Hintergrund: ATF3, or Activating Transcription Factor 3, is a member of mammalian activation TF/CREB protein family of transcription factors. ATF3 binds the cAMP response element (cre) (consensus: 5'-gtgacgt[ac][ag]-3'), a sequence present in many viral and cellular promoters. However, ATF3 represses rather than activates transcription from promoters with ATF sites stabilizing inhibitory co-factors at the promoter. Alternate splicing forms of ATF3, called ATF3 delta Zip, lack the leucine zipper domain and do not bind DNA. ATF3 delta Zip stimulates transcription, presumably by sequestering inhibitory co-factors away from the promoter. Human ATF3 is a 20575 Da protein composed of 181 amino acids.Synonyms: ATF-3, Activating transcription factor 3, Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3

Gen-ID: 467

NCBI Accession: NP_001025458

UniProt: P18847

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response