GRP94 Antikörper (Internal Region)

Produktnummer ABIN374699

Produktdetails anti-GRP94 Antikörper

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein, Zebrafisch (Danio rerio)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GRP94 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: C-KEGVKFDESE KTKE

Spezifität: This antibody recognizes GPR94/TRA1 (Internal).

Kreuzreaktivität (Details): Species reactivity (expected):Canine (Dog), Pig, Cow and Zebrafish.
Species reactivity (tested):Human, Mouse, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_003290.1. Genename: HSP90B1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/32000. Western blot: 0.01-0.03 μg/mL. Approx 100 kDa band observed in lysates of cell lineNIH/3T3 (calculated MW of 92.0 kDa according to NP_003290.1). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Liver shows staining insome hepatocyte nuclei and weak cytoplasm staining of hepatocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Andere Bezeichnung: Endoplasmin / HSP90B1 / TRA1 (HSP90B1 Produkte)

Synonyme: ecgp antikoerper, gp96 antikoerper, tra1 antikoerper, grp94 antikoerper, DDBDRAFT_0206450 antikoerper, DDBDRAFT_0215015 antikoerper, DDB_0206450 antikoerper, DDB_0215015 antikoerper, TRA1 antikoerper, HSP90B1 antikoerper, ERp99 antikoerper, GRP94 antikoerper, TA-3 antikoerper, Targ2 antikoerper, Tra-1 antikoerper, Tra1 antikoerper, endoplasmin antikoerper, GP96 antikoerper, fb61d09 antikoerper, tra-1 antikoerper, wu:fb61d09 antikoerper, wu:fq25g01 antikoerper, Grp94 antikoerper, ECGP antikoerper, Gp96 antikoerper, ppk98 antikoerper, hsp108 antikoerper, heat shock protein 90kDa beta (Grp94), member 1 antikoerper, heat shock protein Hsp90 family protein antikoerper, 94 kD glucose-regulated protein antikoerper, heat shock protein 90 beta family member 1 antikoerper, heat shock protein 90, beta (Grp94), member 1 antikoerper, heat shock protein 90, beta (grp94), member 1 antikoerper, heat shock protein 90kDa beta family member 1 L homeolog antikoerper, hsp90b1 antikoerper, grp94 antikoerper, HSP90B1 antikoerper, Hsp90b1 antikoerper, hsp90b1.L antikoerper

Hintergrund: Glucose regulated protein 94 (GRP 94) is a resident protein of the endoplasmic reticulum (ER) and is induced by the accumulation of unfolded proteins suggesting that it might associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP 94 and other resident ER proteins including GRP 78 and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. GRP 94 is also a low affinity, high capacity calcium binding protein, though it's role, if any, in calcium regulation is not understood.Synonyms: 94 kDa glucose-regulated protein, GRP94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1

Gen-ID: 7184

NCBI Accession: NP_003290

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades