Aktivieren Sie Javascript in Ihrem Browser, um unsere Webseite optimal zu nutzen.

GRP94 Antikörper (C-Term)

Name: GRP94 Antikörper (C-Term)
Brand: antibodies-online
SKU: ABIN2855586
Price: 630.63 EUR
Availability: InStock

HSP90B1 Reaktivität: Human, Maus, Ratte WB, IF, IP, ICC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

WB Image Various whole cell extracts (30 μg) were separated by 7.

IHC-P Image Immunohistochemical analysis of paraffin-embedded HEP3B xenograft, using GRP94, antibody at 1:100 dilution.

IHC

ICC/IF Image GRP94 antibody detects GRP94 protein at cytoplasm by immunofluorescent analysis.

WB Image GRP94 antibody detects GRP94 protein by western blot analysis.

IP Image Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody, Western blot analysis was performed using GRP94 antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 7.

WB Image Sample (30 ug of whole cell lysate) A:NIH-3T3 7.

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GRP94, antibody (green) at 1:500 dilution.

8 Abbildungen

(1 reference)

Produktnummer ABIN2855586

Datenblatt (PDF)

Target Alle GRP94 (HSP90B1) Antikörper anzeigen

GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
Bindungsspezifität
19

17

16

7

7

7

7

7

6

4

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reaktivität
130

84

54

39

35

31

30

30

28

28

26

26

25

17

10

2

2

1

1
Human, Maus, Ratte
Wirt
91

39

22

2

1
Kaninchen
Passende Sekundärantikörper
Klonalität
92

63
Polyklonal
Konjugat
69

12

11

11

9

8

4

4

4

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser GRP94 Antikörper ist unkonjugiert
Applikation
147

82

66

62

49

43

36

20

13

13

12

11

4

4

2

2

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität

Hund, Affe, Schwein

Kreuzreaktivität (Details)

Dog (95 %), Monkey (98 %), Pig (95 %)

Produktmerkmale

Rabbit Polyclonal antibody to GRP94 (heat shock protein 90 kDa beta (Grp94), member 1)
GRP94 antibody

Aufreinigung

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GRP94. The exact sequence is proprietary.

Isotyp

IgG

Top Product

Discover our top product HSP90B1 Primärantikörper
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Center) antibody
HSP90B1 Reaktivität: Human, Maus WB, IF, IP, ICC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 550-650) antibody
HSP90B1 Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Internal Region) antibody
Verified HSP90B1 Reaktivität: Human, Maus WB, IHC, IF, FACS, ELISA Wirt: Ziege Polyclonal unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 676-803) antibody
HSP90B1 Reaktivität: Human WB, IF, IHC (p), EIA Wirt: Maus Monoclonal 2H3 unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 16-43), (N-Term) antibody
HSP90B1 Reaktivität: Human, Maus, Hamster WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB25612 unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibody
HSP90B1 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Huhn, Hamster, Hund, Kaninchen, Schwein, Meerschweinchen, Affe, Schaf, Pferd, Xenopus laevis WB, IHC, IF, FACS, StM Wirt: Ratte Monoclonal 9G10-F8-2 unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibody
HSP90B1 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Huhn, Hamster, Hund, Kaninchen, Schwein, Meerschweinchen, Affe, Schaf, Pferd, Xenopus laevis IHC, FACS, StM Wirt: Kaninchen Monoclonal HSP90B1-3168R unconjugated Recombinant Antibody

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibody
HSP90B1 Reaktivität: Human, Maus, Ratte WB, IHC, IF, ELISA, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibody
HSP90B1 Reaktivität: Human WB, IHC, IF, ELISA, IP Wirt: Maus Monoclonal H9010 unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 460-487) antibody
HSP90B1 Reaktivität: Human WB, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB31597 unconjugated

Applikationshinweise

Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Kommentare

Positive Control: 293T , A431 , HeLa , HepG2 , NIH-3T3 , Mouse brain , PC-12 , Rat-2

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 mg/mL

Buffer

0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel

Thimerosal (Merthiolate)

Vorsichtsmaßnahmen

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C

Informationen zur Lagerung

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Brzozowa-Zasada, Kurek, Piecuch, Wyrobiec: "The clinical and prognostic evaluation of GRP94 immunoexpression in Caucasian patients with colorectal adenocarcinoma." in: Przeglad gastroenterologiczny, Vol. 14, Issue 2, pp. 140-147, (2019) (PubMed).
Target

GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Andere Bezeichnung

GRP94 (HSP90B1 Produkte)

Synonyme

ecgp antikoerper, gp96 antikoerper, tra1 antikoerper, grp94 antikoerper, DDBDRAFT_0206450 antikoerper, DDBDRAFT_0215015 antikoerper, DDB_0206450 antikoerper, DDB_0215015 antikoerper, TRA1 antikoerper, HSP90B1 antikoerper, ERp99 antikoerper, GRP94 antikoerper, TA-3 antikoerper, Targ2 antikoerper, Tra-1 antikoerper, Tra1 antikoerper, endoplasmin antikoerper, GP96 antikoerper, fb61d09 antikoerper, tra-1 antikoerper, wu:fb61d09 antikoerper, wu:fq25g01 antikoerper, Grp94 antikoerper, ECGP antikoerper, Gp96 antikoerper, ppk98 antikoerper, hsp108 antikoerper, heat shock protein 90kDa beta (Grp94), member 1 antikoerper, heat shock protein Hsp90 family protein antikoerper, 94 kD glucose-regulated protein antikoerper, heat shock protein 90 beta family member 1 antikoerper, heat shock protein 90, beta (Grp94), member 1 antikoerper, heat shock protein 90, beta (grp94), member 1 antikoerper, heat shock protein 90kDa beta family member 1 L homeolog antikoerper, hsp90b1 antikoerper, grp94 antikoerper, HSP90B1 antikoerper, Hsp90b1 antikoerper, hsp90b1.L antikoerper

Hintergrund

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1, MIM 140571) and mitochondria (TRAP1, MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Cellular Localization: Endoplasmic reticulum lumen , Melanosome

Molekulargewicht

92 kDa

Gen-ID

7184

Pathways

Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades