Dynactin 1 Antikörper (C-Term)

Produktnummer ABIN374269

Produktdetails anti-Dynactin 1 Antikörper

Target: Dynactin 1 (DCTN1)

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Dynactin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequenz: C-QEQLHQLHSR LIS

Spezifität: This antibody is expected to recognise all reported isoforms of Dynactin subunit 1 (NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1).

Kreuzreaktivität (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Human, Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1. Genename: DCTN1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 128000. Western Blot: 0.5-2 μg/mL. pprox 150 kDa band observed in cell lline A549 and HumanTestis lysates (calculated MW of 140 kDa according to NP_004073.2). Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Cerebellum showspixulate staining of cytoplasm in neuronal cells. In paraffin embedded Human Testisshows cytoplasm staining in cells of the seminiferous tubules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Dynactin 1

Target: Dynactin 1 (DCTN1)

Andere Bezeichnung: Dynactin Subunit 1 (DCTN1 Produkte)

Synonyme: DAP-150 antikoerper, DP-150 antikoerper, P135 antikoerper, Chip150 antikoerper, DCTN1 antikoerper, DKFZp469C1813 antikoerper, AL022633 antikoerper, Glued antikoerper, p150 antikoerper, p150 antikoerper, CG9206 antikoerper, DYNA_DROME antikoerper, Dmel\\CG9206 antikoerper, FCP-D antikoerper, Glu antikoerper, P150 antikoerper, P150[Glued] antikoerper, p150/Glued antikoerper, p150/glued antikoerper, p150[Glued] antikoerper, p150[glued] antikoerper, t1 antikoerper, Dynactin-1 antikoerper, glued antikoerper, dynactin subunit 1 antikoerper, dynactin 1 antikoerper, Dynactin 1, p150 subunit antikoerper, dynactin subunit 1 L homeolog antikoerper, DCTN1 antikoerper, Dctn1 antikoerper, DCTN1-p150 antikoerper, dctn1.L antikoerper

Hintergrund: The DCTN1 gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein. Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.Synonyms: 150 kDa dynein-associated polypeptide, DAP-150, DCTN1, DP-150, p135, p150-glued

Gen-ID: 1639

NCBI Accession: NP_004073

UniProt: Q14203

Pathways: M Phase, ER-Nucleus Signaling