Collagen IV Antikörper

Produktnummer ABIN372918

Produktdetails anti-Collagen IV Antikörper

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reaktivität: Human, Rind (Kuh), Säugetier

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen IV Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Spezifität: This antibody reacts with most Mammalian Type IV Collagens and has negligible cross-reactivity with type I, II, III, V, or VI Collagens.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Mammal.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Collagen Type IV from Human and Bovine placenta.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot. Immunoprecipitation. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL. Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 with 0.01 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to opening. This product is stable at 2-8 °C as an undiluted liquid. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at-20 °C or below. Dilute only prior to immediate use.

Details zu Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: Collagen Type IV (COL4 Produkte)

Synonyme: collagen, type IV antikoerper, Let-2 antikoerper

Hintergrund: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.Synonyms: COL4A1

Gen-ID: 1282

NCBI Accession: NP_001836

UniProt: P02462