CD25 Antikörper

Produktnummer ABIN370891

Produktdetails anti-CD25 Antikörper

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD25 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Spezifität: This antibody is specific to Low affinity alpha chain IL-2 Receptor (IL-2Ra), Mr 55 kDa. The 3C7 monoclonal antibody reacts with an epitope of CD25 which is distinct from that recognized by clone 7D4. (Ref.2,11,12) Clone 3C7, used in combination with 7D4 in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone. (Ref.2,13)

Produktmerkmale: Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TACantigen

Endotoxin-Niveau: Low Endotoxin

Klon: 3C7

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Identification and enumeration of CD25+ (Ref.1-4)Immunoprecipitation. (Ref.1,2,8,10)Functional Assay: Inhibition of IL-2 or mitogen-induced T cell proliferation. (Ref.13)In vitro blocking of IL-2 binding. (Ref.2,12)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS containing no preservatives.

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: CD25 / IL2RA (IL2RA Produkte)

Synonyme: CD25 antikoerper, IDDM10 antikoerper, IL2R antikoerper, TCGFR antikoerper, IL2RAC antikoerper, IL-2RA antikoerper, Il2r antikoerper, Ly-43 antikoerper, interleukin 2 receptor subunit alpha antikoerper, interleukin-2 receptor subunit alpha-like antikoerper, interleukin 2 receptor, alpha chain antikoerper, IL2RA antikoerper, Il2ra antikoerper, LOC100222957 antikoerper

Hintergrund: The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. (Ref.14) CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. (Ref.2,3,15) Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated. (Ref.4) CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD. (Ref.5) It is expressed at a higher level on CD4+CD8+ thymocytes. (Ref.5,6) It is also expressed on cultured epidermal Langerhans cells. (Ref.7) The biochemical features of murine CD25 have been characterized in detail. (Ref.8-10)Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

Gen-ID: 16184

UniProt: P01590

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation