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CD25 Antikörper (AA 34-139)

Name: CD25 Antikörper (AA 34-139)
Brand: antibodies-online
SKU: ABIN1724861
Price: 614.71 EUR
Availability: InStock

IL2RA Reaktivität: Human, Maus, Ratte, Affe WB, IHC, ELISA, ICC Wirt: Maus Monoclonal 1B5D12 unconjugated

Western blot analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng),

ELISA

Immunofluorescence analysis of Hela cells using IL2RA mouse mAb (green).

Western blot analysis using IL2RA mAb against human IL2RA recombinant protein.

Western blot analysis using IL2RA mAb against HEK293 (1) and IL2RA (AA: 34-139)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using IL2RA mouse mAb with DAB staining.

IHC

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IL2RA mouse mAb with DAB staining.

IHC

7 Abbildungen

(2 Referenzen)

Produktnummer ABIN1724861

Datenblatt (PDF)

Target Alle CD25 (IL2RA) Antikörper anzeigen

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
Bindungsspezifität
16

10

6

6

5

5

4

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 34-139
Reaktivität
186

126

37

9

6

3

2

2

2

1

1

1
Human, Maus, Ratte, Affe
Wirt
148

98

80

2

1
Maus
Passende Sekundärantikörper
Klonalität
248

77

2
Monoklonal
Konjugat
112

34

29

27

22

11

8

8

6

6

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD25 Antikörper ist unkonjugiert
Applikation
223

90

56

49

43

41

22

18

14

13

10

9

7

6

4

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Aufreinigung

purified

Immunogen

Purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E. coli.

Klon

1B5D12

Isotyp

IgG1

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anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody
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Applikationshinweise

ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C/-20 °C

Informationen zur Lagerung

4°C, -20°C for long term storage
Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: "Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients." in: Annals of hematology, Vol. 91, Issue 10, pp. 1597-602, (2012) (PubMed).

Sun, Wang, Zhu, Fang, Yu, Xu: "The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses." in: Transplantation proceedings, Vol. 44, Issue 4, pp. 1139-42, (2012) (PubMed).
Target

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung

IL2RA (IL2RA Produkte)

Synonyme

CD25 antikoerper, IDDM10 antikoerper, IL2R antikoerper, TCGFR antikoerper, IL2RAC antikoerper, IL-2RA antikoerper, Il2r antikoerper, Ly-43 antikoerper, interleukin 2 receptor subunit alpha antikoerper, interleukin-2 receptor subunit alpha-like antikoerper, interleukin 2 receptor, alpha chain antikoerper, IL2RA antikoerper, Il2ra antikoerper, LOC100222957 antikoerper

Hintergrund

Description: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. , , , ,
Aliases: CD25, IL2R, TCGFR, IDDM10

Molekulargewicht

30.8 kDa

Gen-ID

3559

HGNC

3559

Pathways

JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation