PPP2CA Antikörper (N-Term)

Produktnummer ABIN3044492

Produktdetails anti-PPP2CA Antikörper

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Bindungsspezifität: AA 6-20, N-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PPP2CA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PPP2CA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: FTKELDQWIE QLNEC

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PPP2CA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: protein phosphatase 2, catalytic subunit, alpha isozyme
Protein Name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PP2A-alpha)

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PP2A-alpha (6-20aa FTKELDQWIEQLNEC), identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-PPP2CA Antikörper (ABIN3044492)

Tian, Qiu, Zhao, Li, Guo: "Sphingomyelinase-induced ceramide production stimulate calcium-independent JNK and PP2A activation following cerebral ischemia." in: Brain injury, Vol. 23, Issue 13-14, pp. 1073-80, (2009) (PubMed).

Details zu PPP2CA

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Andere Bezeichnung: PPP2CA (PPP2CA Produkte)

Synonyme: PP2Ac antikoerper, PP2CA antikoerper, PP2Calpha antikoerper, RP-C antikoerper, PP2A antikoerper, R75353 antikoerper, Pp2a1 antikoerper, pp2ac antikoerper, zgc:110641 antikoerper, protein phosphatase 2 catalytic subunit alpha antikoerper, protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform antikoerper, protein phosphatase 2, catalytic subunit, alpha isozyme antikoerper, protein phosphatase 2 catalytic subunit alpha L homeolog antikoerper, protein phosphatase 2, catalytic subunit, beta isozyme antikoerper, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antikoerper, PPP2CA antikoerper, Ppp2ca antikoerper, ppp2ca.L antikoerper, ppp2cb antikoerper, LOC471633 antikoerper, ppp2ca antikoerper

Hintergrund: The catalytic subunit of human protein phosphatase 2A(PPP2CA) encodes a 309-amino acid polypeptide.It is localized to chromosome 5. The gene(approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue(Leu309) of protein serine/threonine phosphatase 2A catalytic subunit(PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.

Synonyms: PP2A alpha antibody|PP2A-alpha antibody|PP2AA_HUMAN antibody|PP2Aalpha antibody|PP2Ac antibody|PP2CA antibody|PP2Calpha antibody|PPP2CA antibody|Protein phosphatase 2(formerly 2A) catalytic subunit alpha isoform antibody|Protein phosphatase 2 catalytic subunit alpha isoform antibody|Protein phosphatase 2, catalytic subunit, alpha isozyme antibody|Protein phosphatase 2A catalytic subunit, alpha isoform antibody|Replication protein C antibody|RP C antibody|RP-C antibody|RPC antibody|Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody|Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody

UniProt: P67775

Pathways: PI3K-Akt Signalweg, Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades