PPP2CA Antikörper (AA 1-309)

Produktnummer ABIN3043356

Produktdetails anti-PPP2CA Antikörper

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Bindungsspezifität: AA 1-309

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PPP2CA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PPP2CA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Kreuzreaktivität (Details): PP2A-alpha shares 100 % cross reactivity with PPP2CB.

Produktmerkmale: Rabbit IgG polyclonal antibody for Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PPP2CA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: protein phosphatase 2, catalytic subunit, alpha isozyme
Protein Name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PP2A-alpha recombinant protein (Position: M1-L309). Human PP2A-alpha shares 100% amino acid (aa) sequence identity with both mouse and rat PP2A-alpha.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for PP2A-alpha is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu PPP2CA

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Andere Bezeichnung: PPP2CA (PPP2CA Produkte)

Synonyme: PP2Ac antikoerper, PP2CA antikoerper, PP2Calpha antikoerper, RP-C antikoerper, PP2A antikoerper, R75353 antikoerper, Pp2a1 antikoerper, pp2ac antikoerper, zgc:110641 antikoerper, protein phosphatase 2 catalytic subunit alpha antikoerper, protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform antikoerper, protein phosphatase 2, catalytic subunit, alpha isozyme antikoerper, protein phosphatase 2 catalytic subunit alpha L homeolog antikoerper, protein phosphatase 2, catalytic subunit, beta isozyme antikoerper, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antikoerper, PPP2CA antikoerper, Ppp2ca antikoerper, ppp2ca.L antikoerper, ppp2cb antikoerper, LOC471633 antikoerper, ppp2ca antikoerper

Hintergrund: The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide. It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.

Synonyms: PP2A alpha antibody|PP2A-alpha antibody|PP2AA_HUMAN antibody|PP2Aalpha antibody|PP2Ac antibody|PP2CA antibody|PP2Calpha antibody|PPP2CA antibody|Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform antibody|Protein phosphatase 2 catalytic subunit alpha isoform antibody|Protein phosphatase 2, catalytic subunit, alpha isozyme antibody|Protein phosphatase 2A catalytic subunit, alpha isoform antibody|Replication protein C antibody|RP C antibody|RP-C antibody|RPC antibody|Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody|Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody

Gen-ID: 5515

UniProt: P67775

Pathways: PI3K-Akt Signalweg, Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades