CD25 Antikörper (N-Term)

Produktnummer ABIN3044139

Produktdetails anti-CD25 Antikörper

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Bindungsspezifität: AA 39-57, N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Interleukin-2 receptor subunit alpha(IL2RA) detection. Tested with WB, IHC-P in Human.

Sequenz: MAYKEGTMLN CECKRGFRR

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Interleukin-2 receptor subunit alpha(IL2RA) detection. Tested with WB, IHC-P in Human.
Gene Name: interleukin 2 receptor, alpha
Protein Name: Interleukin-2 receptor subunit alpha(IL-2 receptor subunit alpha/IL-2-RA/IL-2R subunit alpha/IL2-RA)

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CD25/IL-2sR Alpha(39-57aa MAYKEGTMLNCECKRGFRR).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: IL2RA (IL2RA Produkte)

Synonyme: CD25 antikoerper, IDDM10 antikoerper, IL2R antikoerper, TCGFR antikoerper, IL2RAC antikoerper, IL-2RA antikoerper, Il2r antikoerper, Ly-43 antikoerper, interleukin 2 receptor subunit alpha antikoerper, interleukin-2 receptor subunit alpha-like antikoerper, interleukin 2 receptor, alpha chain antikoerper, IL2RA antikoerper, Il2ra antikoerper, LOC100222957 antikoerper

Hintergrund: IL2RA(Interleukin 2 receptor, alpha), also called IL2R, IL2R, Alpha Chain or CD25, is a protein that in humans is encoded by the IL2RA gene.The IL2RA gene is mapped on 10p15.1. IL2RA is composed of strand-swapped "sushi-like" domains, unlike the classical cytokine receptor fold. As a result of this domain swap, IL2RA uses a composite surface to dock into a groove on IL2 that also serves as a binding site for antagonist drugs.Homodimeric alpha chains(IL2RA) result in low-affinity receptor, while homodimeric beta(IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Infection by the protozoan Trypanosoma cruzi causes Chagas disease, characterized by a reduction in the amount of IL2RA expressed on the surface of immune cells. This leads to chronic immune suppression, becoming increasingly severe over the course of many years and ultimately resulting in death if left untreated.

Synonyms: CD25 antibody|IDDM10 antibody|IL 2 RA antibody|IL 2 receptor subunit alpha antibodyIL 2R alpha chain antibody|IL 2R subunit alpha antibody|IL-2 receptor subunit alpha antibody|IL-2-RA antibody|IL-2R subunit alpha antibody|IL2 RA antibody|IL2 Receptor alpha antibody|IL2 receptor subunit alpha antibody|IL2-RA antibody|IL2R alpha chain antibody|IL2R antibody|IL2R subunit alpha antibody|IL2RA antibody|IL2RA_HUMAN antibody|Interleukin 2 receptor alpha chain antibody|interleukin 2 receptor subunit alpha antibody|Interleukin 2 receptor, alpha antibody|Interleukin-2 receptor subunit alpha antibody|Ly 43 antibody|Ly43 antibody|OTTHUMP00000019030 antibody|OTTHUMP00000019031 antibody|OTTHUMP00000019032 antibody|p55 antibody|p55 chain antibody|T Cell Growth Factor Receptor antibody|TAC antigen antibody|TCGFR antibody

UniProt: P01589

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation