Dieser Angiotensin I Converting Enzyme 1 Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Aufreinigung
Antigen affinity
Immunogen
An amino acid sequence from the middle region of human Angiotensin Converting Enzyme 1 (TQARKFDVNQLQNT) was used as the immunogen for this ACE antibody.
ACE
Reaktivität: Human
WB, ELISA, IF
Wirt: Maus
Monoclonal
4B10
unconjugated
Applikationshinweise
The stated application concentrations are suggested starting amounts. Titration of the ACE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Lagerung
-20 °C
Informationen zur Lagerung
After reconstitution, the ACE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Target
Angiotensin I Converting Enzyme 1 (ACE)
(Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))
Angiotensin converting enzyme, also called DCP or CD143, is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is though to play a critical role in blood pressure regulation. The ACE gene is mapped to 17q23.3. This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies.