Dieser Topoisomerase II alpha Antikörper ist unkonjugiert
Applikation
Western Blotting (WB)
Aufreinigung
Antigen affinity
Immunogen
An amino acid sequence from the C-terminus of human Topoisomerase II alpha (RKPSTSDDSDSNFEK) was used as the immunogen for this Topoisomerase II alpha antibody.
TOP2A
Reaktivität: Human
WB, ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
The stated application concentrations are suggested starting amounts. Titration of the Topoisomerase II alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Lagerung
-20 °C
Informationen zur Lagerung
After reconstitution, the Topoisomerase II alpha antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Target
Topoisomerase II alpha (TOP2A)
(Topoisomerase (DNA) II alpha 170kDa (TOP2A))
TOP2 antikoerper, TP2A antikoerper, TOP2A antikoerper, Top-2 antikoerper, TOP-2 antikoerper, F12B17.110 antikoerper, F12B17_110 antikoerper, thimet metalloendopeptidase 2 antikoerper, fc08c07 antikoerper, wu:fc08c07 antikoerper, DNA topoisomerase II alpha antikoerper, DNA topoisomerase II, alpha isozyme antikoerper, topoisomerase (DNA) II alpha antikoerper, Zincin-like metalloproteases family protein antikoerper, TOP2A antikoerper, Bm1_08705 antikoerper, Top2a antikoerper, AT5G10540 antikoerper, top2a antikoerper
Hintergrund
The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22. The TOP2A gene spans approximately 30 kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 Molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1 % of the nuclear extract. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II.