Cyclin D1 Antikörper

Produktnummer ABIN3026635

Produktdetails anti-Cyclin D1 Antikörper

Target: Cyclin D1 (CCND1)

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin D1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktmerkmale: This antibody recognizes a protein of 36 kDa, identified as Cyclin D1. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms and a key cell cycle regulator. This antibody neutralizes the activity of Cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression. Cyclin D1 antibody is useful in identifying mantle cell lymphomas, which stain positive, from CLL/SLL and follicular lymphomas, which stain negative. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Aufreinigung: Protein G affinity chromatography

Immunogen: Human recombinant full length protein was used as the immunogen for this Cyclin D1 antibody.

Klon: DCS-6

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin D1 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Cyclin D1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Cyclin D1

Target: Cyclin D1 (CCND1)

Andere Bezeichnung: Cyclin D1 (CCND1 Produkte)

Synonyme: BCL1 antikoerper, D11S287E antikoerper, PRAD1 antikoerper, U21B31 antikoerper, AI327039 antikoerper, CycD1 antikoerper, Cyl-1 antikoerper, bcl-1 antikoerper, cD1 antikoerper, cb161 antikoerper, cycd1 antikoerper, etID37810.7 antikoerper, fb52e01 antikoerper, fc45c08 antikoerper, fc83a12 antikoerper, id:ibd1198 antikoerper, wu:fb52e01 antikoerper, wu:fc45c08 antikoerper, wu:fc83a12 antikoerper, ccnd1b antikoerper, prad1 antikoerper, ccnd1 antikoerper, ccnd1a antikoerper, bcl1 antikoerper, d11s287e antikoerper, u21b31 antikoerper, CCND1 antikoerper, cyclin D1 antikoerper, cyclin D1 S homeolog antikoerper, cyclin D1 L homeolog antikoerper, CCND1 antikoerper, Ccnd1 antikoerper, ccnd1 antikoerper, ccnd1.S antikoerper, ccnd1.L antikoerper

Hintergrund: This antibody recognizes a protein of 36  kDa, identified as Cyclin D1. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms and a key cell cycle regulator. This antibody neutralizes the activity of Cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression. Cyclin D1 antibody is useful in identifying mantle cell lymphomas, which stain positive, from CLL/SLL and follicular lymphomas, which stain negative. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Gen-ID: 595

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, ER-Nucleus Signaling