Collagen IV Antikörper

Produktnummer ABIN289410

Produktdetails anti-Collagen IV Antikörper

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen IV Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Produktmerkmale: Affinity purified Rabbit polyclonal Collagen Type IV antibody
Bioactivity: Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues

Aufreinigung: Affinity chromatography purified

Sterilität: Filtered

Immunogen: Collagen type IV antibody was raised in rabbit using collagen type IV from human and bovine placenta as the immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1:5,000-1:20,000, IHC: 1:50-1:200, WB: 1:500-1:5,000
Optimal conditions should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Sterile filtered liquid in 0.125 M Na5 5 7, pH 8.0, with 0.075 NaCl, 0.005 EDTA, and 0.01 % NaN3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Referenzen für anti-Collagen IV Antikörper (ABIN289410)

Tane, Dhar, Roy, Pinheiro, Ohira, Roy: "Effect of excess synthesis of extracellular matrix components by trabecular meshwork cells: possible consequence on aqueous outflow." in: Experimental eye research, Vol. 84, Issue 5, pp. 832-42, (2007) (PubMed).

Details zu Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: Collagen Type IV (COL4 Produkte)

Synonyme: collagen, type IV antikoerper, Let-2 antikoerper

Hintergrund: Collagen is a group of naturally occurring proteins found, in nature, exclusively in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content. Type-IV collagen is a type of collagen found primarily in the basal lamina. The C-terminus domain is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, type-IV lacks the regular glycine in every third residue necessary for the tight, collagen helix.