EGF Antikörper (Extracellular Domain)

Produktnummer ABIN2620256

Produktdetails anti-EGF Antikörper

Target: EGF (Epidermal Growth Factor (EGF))

Bindungsspezifität: AA 106-119, Extracellular Domain

Reaktivität: Human, Kaninchen, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EGF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human EGF. This antibody is expected to recognize all reported isoforms (NP_001954.2, NP_001171601.1, NP_001171602.1), but not the mature form. The immunizing peptide represents part of the extracellular domain.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide SRQERVCNIEKNVS from an internal region (near the N-terminus) of human EGF (NP_001954.2, NP_001171601.1, NP_001171602.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Rabbit (100%), Ferret (93%), Bat, Guinea pig (86%), Rat (57%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 160kD double band observed in Human Prostate lysates (calculated MW of 134kD according to NP_001954.2). The two bands represent glycosylated forms of the immature protein. Recommended concentration: 1-3 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF (EGF Produkte)

Synonyme: HOMG4 antikoerper, URG antikoerper, AI790464 antikoerper, CEGF antikoerper, epidermal growth factor antikoerper, pro-epidermal growth factor antikoerper, EGF antikoerper, egf antikoerper, CpipJ_CPIJ020278 antikoerper, Egf antikoerper

Hintergrund: Name/Gene ID: EGF

Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor

Gen-ID: 1950

NCBI Accession: NP_001954, NP_001171601, NP_001171602

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation