ATF3 Antikörper

Produktnummer ABIN2458249

Produktdetails anti-ATF3 Antikörper

Target: ATF3 (Activating Transcription Factor 3 (ATF3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATF3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Aufreinigung: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATF3.

Anwendungsinformationen

Applikationshinweise: ATF3 antibody can be used for detection of ATF3 by ELISA at 1:312500. ATF3 antibody can be used for detection of ATF3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Konzentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handhabung: As with any antibody avoid repeat freeze-thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ATF3 antibody at -20 °C.

Details zu ATF3

Target: ATF3 (Activating Transcription Factor 3 (ATF3))

Andere Bezeichnung: ATF3 (ATF3 Produkte)

Synonyme: zgc:55526 antikoerper, ATF3 antikoerper, LRG-21 antikoerper, LRF-1 antikoerper, LRFI antikoerper, atf-3 antikoerper, activating transcription factor 3 antikoerper, activating transcription factor 3 L homeolog antikoerper, atf3 antikoerper, ATF3 antikoerper, Atf3 antikoerper, atf3.L antikoerper

Hintergrund: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. ATF3 represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.

Molekulargewicht: 20 kDa

Gen-ID: 467

NCBI Accession: NP_001665

UniProt: P18847

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response