c-MET Antikörper (pTyr1249, pTyr1356)
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- Target Alle c-MET (MET) Antikörper anzeigen
- c-MET (MET) (Met Proto-Oncogene (MET))
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Bindungsspezifität
- pTyr1249, pTyr1356
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser c-MET Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA
- Spezifität
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using dual-phospho-peptide coupled to agarose beads followed by cross-adsorption against nonphospho-peptide. This antibody is specific for human c-Met protein phosphorylated at Y1349 and Y1356. A BLAST analysis was used to suggest cross-reactivity with c-Met from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with c-Met from other sources has not been determined.
- Kreuzreaktivität
- Maus, Ratte (Rattus)
- Produktmerkmale
- This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-c-MET is the receptor for hepatocyte growth factor (also known as scatter factor, HGF/SF), and belongs to the tyrosine kinase superfamily. Interaction of c-Met with HGF results in autophosphorylation of c-Met at multiple tyrosines. Phosphorylation of Y1234/1235 in the c-Met kinase domain is critical to kinase activation. When phosphorylated, Y1349 and Y1356, along with surrounding amino acids, form a unique bidentate docking site for substrates such as Gab1, Grb2, phosphatidylinositol 3-kinase (PI3K) and others. C-Met mainly uses the Gab1 scaffolding adaptor in its initial step of signal transmission. Well-characterized downstream signalling pathways that are activated by c-Met include the ERK/MAPK, PI3K-Akt/PKB, Crk-Rap and Rac-Pak pathways, resulting in proliferation and increased cell survival. Anti-Phospho cMET was developed with NCI and is ideal for Cancer Research.
- Aufreinigung
- affinity purified
- Sterilität
- Sterile filtered
- Immunogen
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This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1349 and Y1356 of human c-Met protein.
Immunogen Type: Peptide - Isotyp
- IgG
- Top Product
- Discover our top product MET Primärantikörper
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- Applikationshinweise
- This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 150 kDa in size corresponding to phosphorylated c-Met protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human c-Met pY1349pY1356 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
- Kommentare
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Gene Name: MET
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1.01 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.
- Haltbarkeit
- 3 months
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- Target
- c-MET (MET) (Met Proto-Oncogene (MET))
- Andere Bezeichnung
- C-Met (MET Produkte)
- Synonyme
- AUTS9 antikoerper, HGFR antikoerper, RCCP2 antikoerper, c-Met antikoerper, AI838057 antikoerper, HGF antikoerper, Par4 antikoerper, Hgfr antikoerper, c-met antikoerper, MET antikoerper, C-MET antikoerper, met antikoerper, met-A antikoerper, MET proto-oncogene, receptor tyrosine kinase antikoerper, met proto-oncogene antikoerper, MET proto-oncogene, receptor tyrosine kinase L homeolog antikoerper, MET antikoerper, Met antikoerper, met.L antikoerper
- Hintergrund
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This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-c-MET is the receptor for hepatocyte growth factor (also known as scatter factor, HGF/SF), and belongs to the tyrosine kinase superfamily. Interaction of c-Met with HGF results in autophosphorylation of c-Met at multiple tyrosines. Phosphorylation of Y1234/1235 in the c-Met kinase domain is critical to kinase activation. When phosphorylated, Y1349 and Y1356, along with surrounding amino acids, form a unique bidentate docking site for substrates such as Gab1, Grb2, phosphatidylinositol 3-kinase (PI3K) and others. C-Met mainly uses the Gab1 scaffolding adaptor in its initial step of signal transmission. Well-characterized downstream signalling pathways that are activated by c-Met include the ERK/MAPK, PI3K–Akt/PKB, Crk–Rap and Rac–Pak pathways, resulting in proliferation and increased cell survival. Anti-Phospho cMET was developed with NCI and is ideal for Cancer Research.
Synonyms: HGF receptor antibody, HGF/SF receptor antibody, HGFR antibody, MET antibody, Met proto oncogene tyrosine kinase antibody - Gen-ID
- 4233, 42741655
- UniProt
- P08581
- Pathways
- RTK Signalweg, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
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