PCNA Antikörper

Produktnummer ABIN233602

Produktdetails anti-PCNA Antikörper

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human, Maus, Ratte, Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Spezifität: Recognizes a non-histone protein of 36kD, proliferating cell nuclear antigen (PCNA). Species cross-reactivity: Human, mouse, rat, insect and yeast.

Aufreinigung: Protein A purified

Immunogen: Recombinant rat PCNA protein.

Type of Immunogen: Recombinant protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Approved: Flo, IHC, IHC-P, IP, WB (1:25)

Usage: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native and denatured): 2 μg/mg protein lysate. Use Protein A. Western Blot: 1:25 for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT. Requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: Raji cells. Tonsil, lymph node, or small intestine.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide, glycerol to 40 %.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA / Cyclin (PCNA Produkte)

Synonyme: PCNAR antikoerper, Pcna/cyclin antikoerper, pcna antikoerper, AO090003000846 antikoerper, 53/13 antikoerper, CG9193 antikoerper, DmPCNA antikoerper, Dmel\\CG9193 antikoerper, MUS209 antikoerper, Mus209 antikoerper, PCNA antikoerper, PCNA/mus209 antikoerper, Pcna antikoerper, dPCNA antikoerper, l(2)02448 antikoerper, l(2)56F antikoerper, l(2)56Fa antikoerper, l(2)s1534 antikoerper, cb16 antikoerper, fb36g03 antikoerper, wu:fa28e03 antikoerper, wu:fb36g03 antikoerper, pcna-A antikoerper, proliferating cell nuclear antigen antikoerper, DNA polymerase III sliding clamp antikoerper, Proliferating cell nuclear antigen antikoerper, Proliferating cell nuclear antigen-like antikoerper, proliferating cell nuclear antigen L homeolog antikoerper, proliferating cell nuclear antigen 1 antikoerper, PCNA antikoerper, Pcna antikoerper, pcna antikoerper, MMP_RS08810 antikoerper, ANI_1_512014 antikoerper, AOR_1_1500154 antikoerper, MGC53867 antikoerper, pcna.L antikoerper, pcna1 antikoerper, LOC106420145 antikoerper, pcn1 antikoerper

Hintergrund: Name/Gene ID: PCNA

Synonyms: PCNA, Cyclin

Gen-ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie