PCNA Antikörper (AA 151-261)

Produktnummer ABIN676583

Es sind 6+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-PCNA Antikörper wird verwendet zum Nachweis von PCNA in Proben von Human. Er wurde validiert für WB, IHC (p), IF (p), IF (cc) und IHC (fro).

Kurzübersicht für PCNA Antikörper (AA 151-261) (ABIN676583)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-PCNA Antikörper

Bindungsspezifität: AA 151-261

Verwendungszweck: PCNA Polyclonal Antibody

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Cow,Sheep,Pig,Chicken,Rabbit,Guinea Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PCNA

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB(1:300-5000),

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-PCNA Antikörper (ABIN676583)

Zhao, Han, Hong, Sun: "Adipose differentiation‑related protein knockdown inhibits vascular smooth muscle cell proliferation and migration and attenuates neointima formation." in: Molecular medicine reports, Vol. 16, Issue 3, pp. 3079-3086, (2018) (PubMed).

Zhou, Zhang, Gao, Tan, Zheng, Zhao, Zhang: "Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-β1/Smad pathway." in: International journal of molecular medicine, Vol. 41, Issue 1, pp. 473-484, (2018) (PubMed).

Zhao, Feng, Zhang, Kou, Li, Liu, Zhang, Cui, Chu, Shen, Min: "Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production." in: Theriogenology, Vol. 89, pp. 280-288, (2017) (PubMed).

Ji, Liu, Zhao, Guo, Xia, Lu, Yin, Xu: "Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells." in: Molecular medicine reports, Vol. 16, Issue 5, pp. 7391-7397, (2017) (PubMed).

Liang, Peng, Li, Yang, Xie, Li, Du, Zhang: "Silencing of CXCR4 sensitizes triple-negative breast cancer cells to cisplatin." in: Oncotarget, Vol. 6, Issue 2, pp. 1020-30, (2015) (PubMed).

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Hintergrund:

Synonyms: ATLD2, Proliferating cell nuclear antigen, PCNA, Cyclin

Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Gen-ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie