EIF2AK2 Antikörper (pThr446)
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- Target Alle EIF2AK2 Antikörper anzeigen
- EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))
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Bindungsspezifität
- pThr446
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser EIF2AK2 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB)
- Spezifität
- Recognizes human PKR phosphorylated at Thr446, Mr 68kD. A non-specific protein was also detected, Mr ~75kD, which is inhibited by the immunizing peptide.
- Aufreinigung
- Protein A purified
- Immunogen
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Peptide corresponding to amino acids surrounding Thr446 of human PKR.
Type of Immunogen: Synthetic peptide - Isotyp
- IgG
- Top Product
- Discover our top product EIF2AK2 Primärantikörper
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- Applikationshinweise
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Approved: WB (0.5 - 2 μg/mL)
Usage: Suitable for use in Western Blot. Western Blot: 0.5-2.0 μg/mL detects phospho-PKR in RIPA lysates from HeLa cells that had. been stimulated with IFN alpha overnight followed by treatment with calyculin A for 15 minutes. Beadlyte Peptide Specificity Assay: 1:1000-1:5000 dilutions were incubated with a cocktail of. microspheres conjugated to either the phosphorylated or non phosphorylated peptides. Treated HeLa (lane 1) or untreated HeLa (lane2) cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-PKR (0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-PKR (68kD). - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))
- Andere Bezeichnung
- EIF2AK2 / PKR (EIF2AK2 Produkte)
- Synonyme
- PKR antikoerper, pkr antikoerper, EIF2AK2 antikoerper, DKFZp469K1934 antikoerper, EIF2AK1 antikoerper, PRKR antikoerper, 2310047A08Rik antikoerper, 4732414G15Rik antikoerper, AI467567 antikoerper, AI747578 antikoerper, Pkr antikoerper, Prkr antikoerper, Tik antikoerper, eukaryotic translation initiation factor 2 alpha kinase 2 antikoerper, eukaryotic translation initiation factor 2-alpha kinase 2 antikoerper, EIF2AK2 antikoerper, eif2ak2 antikoerper, Eif2ak2 antikoerper
- Hintergrund
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Name/Gene ID: EIF2AK2
Subfamily: Translational Control Kinase
Family: Protein Kinase
Synonyms: EIF2AK2, p1/eIF-2A protein kinase, p68 kinase, PRKR, EIF-2A protein kinase 2, p68, Protein kinase RNA-activated, Tik - Gen-ID
- 5610
- UniProt
- P19525
- Pathways
- DNA Reparatur, ER-Nucleus Signaling, Hepatitis C
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