EIF2AK2 Antikörper (AA 11-42)

Produktnummer ABIN3030884

Der Kaninchen Polyklonal anti-EIF2AK2 Antikörper wird verwendet zum Nachweis von EIF2AK2 in Proben von Human und Maus. Er wurde validiert für WB, IHC und ELISA.

Kurzübersicht für EIF2AK2 Antikörper (AA 11-42) (ABIN3030884)

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF2AK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-EIF2AK2 Antikörper

Bindungsspezifität: AA 11-42

Aufreinigung: Purified

Immunogen: A portion of amino acids 11-42 from the human protein was used as the immunogen for this PKR antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the PKR antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the PKR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu EIF2AK2

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Andere Bezeichnung: PKR

Hintergrund: Interferon-induced, double-stranded RNA-activated protein kinase (PRKR/PKR) is a serine-threonine kinase. Activation by dsRNAs leads to autophosphorylation of PKR and allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2 (EIF2-alpha), leading to the inhibition of protein synthesis. PRKR, which is involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, activates IKK and NFKB, thereby suppressing apoptosis. Apoptosis induced by live pathogenic gram-positive and gram-negative bacteria requirs both TLR4 and PKR, possibly representing a major mechanism for pathogenic bacteria that use specific virulence factors to avoid detection and destruction by the innate immune system.

UniProt: P19525

Pathways: DNA Reparatur, ER-Nucleus Signaling, Hepatitis C