Caspase 3 Antikörper

Produktnummer ABIN187760

Produktdetails anti-Caspase 3 Antikörper

Target: Caspase 3 (CASP3)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: Recognizes pro-caspase-3. Species Reactivity: Human, other species not tested.

Immunogen: Full length recombinant caspase-3.

Anwendungsinformationen

Applikationshinweise: Western blot: Use at 2-10 µg/ml The optimal dilution for a specific application should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 250 µg purified IgG at 1 mg/ml in PBS with 0.05% sodium azide

Lagerung: -20 °C

Details zu Caspase 3

Target: Caspase 3 (CASP3)

Andere Bezeichnung: Caspase-3 (CASP3 Produkte)

Synonyme: CPP32 antikoerper, CPP32B antikoerper, SCA-1 antikoerper, A830040C14Rik antikoerper, AC-3 antikoerper, Apopain antikoerper, CC3 antikoerper, Caspase-3 antikoerper, Lice antikoerper, Yama antikoerper, mldy antikoerper, xcpp32 antikoerper, casp3 antikoerper, zgc:100890 antikoerper, CASP-3 antikoerper, caspase-3 antikoerper, caspase 3 antikoerper, caspase 3 S homeolog antikoerper, caspase 3, apoptosis-related cysteine peptidase a antikoerper, caspase 3, apoptosis-related cysteine peptidase antikoerper, CASP3 antikoerper, Casp3 antikoerper, casp3.S antikoerper, casp3a antikoerper

Hintergrund: Caspase-3 along with caspase 7 and 6 form the group of effector caspases that are responsible for the cleavage of multiple substrates including the cytokeratins, PARP, alpha fodrin, NuMA and others. Caspase-7 occurs in three varient forms. Caspase-3-like activities are required for Fas-mediated apoptosis. However, the role of caspase-1 and caspase-3 in mediating Fas-induced cell death is not clear. Although wild-type, caspase-1(-/-), and caspase-3(-/-) hepatocytes were killed at a similar rate when cocultured with FasL expressing NIH 3T3 cells, caspase-3(-/-) hepatocytes displayed drastically different morphological changes as well as significantly delayed DNA fragmentation. For both wild-type and caspase-1(-/-) apoptotic hepatocytes, typical apoptotic features such as cytoplasmic blebbing and nuclear fragmentation are seen within 6 hr, but neither event was observed for caspase-3(-/-) hepatocytes. In thymocytes apoptotic caspase-3(-/-) thymocytes exhibit similar abnormal morphological changes and delayed DNA fragmentation observed in hepatocytes. Cleavage of various caspase substrates implicates apoptotic events, including gelsolin, fodrin, laminB, and DFF45/ICAD are delayed or absent. The altered cleavage of these key substrates is likely responsible for the aberrant apoptosis observed in both hepatocytes and thymocytes deficient in caspase-3.

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation