SMAD3 Antikörper (pSer425)

Produktnummer ABIN1842543

Produktdetails anti-SMAD3 Antikörper

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Bindungsspezifität: pSer425

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF)

Spezifität: Rabbit Anti-Smad3 Polyclonal Antibody detects endogenous levels of total Smad3 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized non-phosphopeptide derived from human Smad3 around pSer425 (C-S-S-V-SP).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC: 1:50-1:100,
IF: 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.,
Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C for 1 year

Referenzen für anti-SMAD3 Antikörper (ABIN1842543)

Tang, Yamashita, Coussens, Tang, Wang, Li, Deng, Cheng, Zhang: "Ablation of Smurf2 reveals an inhibition in TGF-? signalling through multiple mono-ubiquitination of Smad3." in: The EMBO journal, Vol. 30, Issue 23, pp. 4777-89, (2011) (PubMed).

Details zu SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Andere Bezeichnung: Smad3 (SMAD3 Produkte)

Synonyme: HSPC193 antikoerper, HsT17436 antikoerper, JV15-2 antikoerper, LDS1C antikoerper, LDS3 antikoerper, MADH3 antikoerper, madh3 antikoerper, madh3a antikoerper, smad3 antikoerper, wu:fa99e03 antikoerper, XSmad3 antikoerper, XenMLP antikoerper, Xmad3 antikoerper, madh3-A antikoerper, Madh3 antikoerper, AU022421 antikoerper, Smad 3 antikoerper, mad3 antikoerper, madh3b antikoerper, zgc:92234 antikoerper, SMAD family member 3 antikoerper, SMAD family member 3a antikoerper, SMAD family member 3 L homeolog antikoerper, SMAD family member 3b antikoerper, SMAD3 antikoerper, smad3a antikoerper, smad3.L antikoerper, Smad3 antikoerper, smad3b antikoerper

Pathways: Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie