Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Spezifität
Rabbit Anti-Src (Phospho-Tyr529) Polyclonal Antibody detects endogenous levels of Src only when phosphorylated at tyrosine 529.
Produktmerkmale
Molecular Weight: Predicted Band Size 60 kDa, Observed Band Size 60 kDa
Aufreinigung
The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Synthesized phosphopeptide derived from human Src around pTyr529 (P-Q-YP-Q-P)
Src
Reaktivität: Human
WB, ELISA, IHC, IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western blot: 1:500-1:1,000, IHC: 1:50-1:100, IF: 1:100-1:200
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1 mg/mL
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Avoid repeated freezing and thawing.