WNT1 Antikörper

Produktnummer ABIN155191

Produktdetails anti-WNT1 Antikörper

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WNT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: The antibody recognizes Human WNT-1. Other species not tested.

Aufreinigung: Affinity chromatography employing immobilized hWNT-1 matrix

Immunogen: Highly pure (>98%) recombinant human WNT-1

Anwendungsinformationen

Applikationshinweise: ElisaIndirect: To detect hWNT-1 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hWNT-1. Sandwich: To detect hWNT-1 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human WNT-1 (PP1231B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-1. Western BlotTo detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable for one year from despatch at -20 °C. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Haltbarkeit: 12 months

Details zu WNT1

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Andere Bezeichnung: WNT1 (WNT1 Produkte)

Synonyme: Xint-1 antikoerper, Xwnt1 antikoerper, int-1 antikoerper, int1 antikoerper, wnt-1 antikoerper, wnt1-a antikoerper, Int-1 antikoerper, Wnt-1 antikoerper, sw antikoerper, swaying antikoerper, BMND16 antikoerper, INT1 antikoerper, OI15 antikoerper, Int1 antikoerper, WNT-1 antikoerper, sb:eu647 antikoerper, zgc:194464 antikoerper, zgc:194478 antikoerper, WNT1 antikoerper, Wnt family member 1 antikoerper, Wnt family member 1 L homeolog antikoerper, wingless-type MMTV integration site family, member 1 antikoerper, WNT1 antikoerper, wnt1.L antikoerper, Wnt1 antikoerper, wnt1 antikoerper

Hintergrund: Synonyms: INT-1, INT1, Proto-oncogene protein Wnt-1

Gen-ID: 7471

NCBI Accession: NP_005421

UniProt: P04628

Pathways: WNT Signalweg, Dopaminergic Neurogenesis