Caspase 3 Antikörper (AA 101-112)

Produktnummer ABIN1100424

Produktdetails anti-Caspase 3 Antikörper

Target: Caspase 3 (CASP3)

Bindungsspezifität: AA 101-112

Reaktivität: Human, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Caspase 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Human Caspase 3. This antibody is expected to recognise both reported isoforms (NP_004337.2 and NP_116786.1).

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Hamster (100%) Orangutan, Monkey, Galago, Marmoset, Sheep, Horse (92%) Mouse, Rat, Bat, Dog, Bovine, Panda, Rabbit, Porcine, Guinea pig (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-RDVSKEDHSKRS from an internal region of human Caspase 3 (NP_004337.2, NP_116786.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Hamster (100%), Orangutan, Monkey, Galago, Marmoset, Sheep, Horse (92%), Mouse, Rat, Bat, Dog, Bovine, Panda, Rabbit, Porcine, Guinea pig (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 30kD band observed in lysates of cell line MOLT4 (calculated MW of 31.6kD according to NP_004337.2,). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consistently observed at 65kD. This band was successfully blocked by incubation with the immunizing peptide.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Caspase 3

Target: Caspase 3 (CASP3)

Andere Bezeichnung: CASP3 / Caspase 3 (CASP3 Produkte)

Synonyme: CPP32 antikoerper, CPP32B antikoerper, SCA-1 antikoerper, A830040C14Rik antikoerper, AC-3 antikoerper, Apopain antikoerper, CC3 antikoerper, Caspase-3 antikoerper, Lice antikoerper, Yama antikoerper, mldy antikoerper, xcpp32 antikoerper, casp3 antikoerper, zgc:100890 antikoerper, CASP-3 antikoerper, caspase-3 antikoerper, caspase 3 antikoerper, caspase 3 S homeolog antikoerper, caspase 3, apoptosis-related cysteine peptidase a antikoerper, caspase 3, apoptosis-related cysteine peptidase antikoerper, CASP3 antikoerper, Casp3 antikoerper, casp3.S antikoerper, casp3a antikoerper

Hintergrund: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

Gen-ID: 836

NCBI Accession: NP_004337, NP_116786

UniProt: P42574

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation