IKBKE
Reaktivität: Human
WB, ELISA, IHC
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
IKKε pT501 antibody is suitable for use in ELISA, western blotting, and although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. An 85 kDa band corresponding to human IKKe is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. Researchers should determine optimal titers for other applications.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1.15 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
DeDiego, Nogales, Martinez-Sobrido, Topham: "Interferon-Induced Protein 44 Interacts with Cellular FK506-Binding Protein 5, Negatively Regulates Host Antiviral Responses, and Supports Virus Replication." in: mBio, Vol. 10, Issue 4, (2020) (PubMed).
Sweeney, Mo, Firestein: "Antiviral gene expression in rheumatoid arthritis: role of IKKepsilon and interferon regulatory factor 3." in: Arthritis and rheumatism, Vol. 56, Issue 3, pp. 743-52, (2007) (PubMed).
Target
IKKi/IKKe (IKBKE)
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
zgc:100844 antikoerper, IKBKE antikoerper, MGC130907 antikoerper, IKK-E antikoerper, IKK-i antikoerper, IKKE antikoerper, IKKI antikoerper, AW558201 antikoerper, IKKepsilon antikoerper, Ikki antikoerper, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon antikoerper, inhibitor of nuclear factor kappa B kinase subunit epsilon antikoerper, inhibitor of nuclear factor kappa B kinase subunit epsilon L homeolog antikoerper, inhibitor of kappaB kinase epsilon antikoerper, IKBKE antikoerper, ikbke antikoerper, ikbke.L antikoerper, Ikbke antikoerper
Hintergrund
Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity. IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKepsilon is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFα or IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa. Anti-IKKε pT501 antibody is ideal for investigators involved in NFkappaB and apoptosis reasearch. Synonyms: I kappa B kinase epsilon antibody, IkBKE antibody, IKBKE protein antibody, Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody, Inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, IKKE, IKKI, KIAA0151