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4 | ABIN1106225 | EIA IHC (fro) IF IHC (p) WB | Biotin | Mouse | IgG2a | AA 1-815, Extracellular Domain, N-Term | Anmelden zum Anzeigen | 5G5 | 7 | |
4 | ABIN784145 | EIA IF IHC (p) WB | Goat | IgG | AA 204-224, N-Term | Anmelden zum Anzeigen | Polyclonal | 0 | ||
1 | ABIN5555342 | EIA IF IHC (p) WB | Rabbit | IgG | C-Term | Anmelden zum Anzeigen | Polyclonal | 0 | ||
1 | ABIN5555343 | EIA IF WB | Rabbit | IgG | Center | Anmelden zum Anzeigen | Polyclonal | 0 | ||
1 | ABIN187334 | EIA FACS IHC (fro) IHC (p) WB | Mouse | IgG2a | Anmelden zum Anzeigen | 5G5 | 0 |
General |
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Antigen | Toll-Like Receptor 9 (TLR9) Antikörper |
Epitop | AA 1-815, Extracellular Domain, N-Term Alternativen |
Reaktivität | Hund, Human, Maus Alternativen |
Wirt | Maus Alternativen |
Klonalität (Klon) | |
Konjugat | Dieser TLR9 Antikörper ist konjugiert mit FITC Alternativen |
Applikation |
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternativen
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7 Publikationen vorhanden |
Hersteller | Anmelden zum Anzeigen |
Produktdetails anti-TLR9 AntikörperTarget Details TLR9 Anwendungsinformationen Handhabung Referenzen für anti-TLR9 Antikörper (ABIN1106226) Bilder |
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Spezifität | The monoclonal antibody 5G5 recognizes human Toll-like receptor 9. |
Kreuzreaktivität (Details) | Species reactivity (tested):Human, mouse, canine |
Reinigung | Protein G |
Immunogen | Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc |
Klon | 5G5 |
Isotyp | IgG2a |
Plasmids, Primers & others | |
Target Details TLR9Produktdetails anti-TLR9 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-TLR9 Antikörper (ABIN1106226) Bilder zurück nach oben |
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Antigen | |
Andere Bezeichnung | CD289 / TLR9 (TLR9 Antibody Abstract) |
Hintergrund | Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκB-dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens. Whereas most TLRs are expressed on the cell surface, TLR9 is expressed intracellularly within one or more endosomal compartments and recognizes nucleic acids. TLR9 detects a rather subtle difference in the DNA of vertebrates compared with that of pathogens. Vertebrate genomic DNAs have mostly methylated CpG dinucleotides where bacterial and viral DNAs have unmethylated CpG dinucleotides. TLR9 undergoes relocation from endoplasmic reticulum to CpG-ODN-containing endosomes. In these endosomes TLR9 becomes a functional receptor after proteolytic cleavage. TLR9 exists as a preformed homodimer and CpG-ODN binding promotes its conformational change, bringing the cytoplasmic TIR-like domains close to each other. This allows a recruitment of the key adapter protein MyD88 which initiates a signalling cascade. The only human immune cell types known to constitutively express TLR9 and to be activated by CpG ODN are pDCs and B cells. TLR9 triggering induces an activation phenotype in the B cells and pDCs, characterized by the expression of costimulatory molecules, resistance to apoptosis, and induces Th1-type immune response profiles.Synonyms: Toll-like receptor 9, UNQ5798/PRO19605 |
Gen-ID | 81897 |
NCBI Accession | NP_112455 |
UniProt | Q9EQU3 |
Pathways | TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades |
AnwendungsinformationenProduktdetails anti-TLR9 Antikörper Target Details TLR9 Handhabung Referenzen für anti-TLR9 Antikörper (ABIN1106226) Bilder zurück nach oben |
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Applikations-hinweise | Optimal working dilution should be determined by the investigator. |
Beschränkungen | Nur für Forschungszwecke einsetzbar |
HandhabungProduktdetails anti-TLR9 Antikörper Target Details TLR9 Anwendungsinformationen Referenzen für anti-TLR9 Antikörper (ABIN1106226) Bilder zurück nach oben |
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Konzentration | 0.1 mg/mL |
Buffer | PBS, 1 % bovine serum albumin, 0.02 % sodium azide |
Konservierungs-mittel | Sodium azide |
Vorsichtsmaßnahmen | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Lagerung | 4 °C |
Informationen zur Lagerung | Store at 2 - 8 °C. |
Referenzen für anti-TLR9 Antikörper (ABIN1106226)Produktdetails anti-TLR9 Antikörper Target Details TLR9 Anwendungsinformationen Handhabung Bilder zurück nach oben |
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Allgemeine Veröffentlichungen |
Jeong, Choi, Cho, Lee, Oh, Park, Cho, Lee, Kim, Han, Choi, Chung: "Chronic gastrointestinal symptoms and quality of life in the Korean population." in: World journal of gastroenterology : WJG, Vol. 14, Issue 41, pp. 6388-94, 2008 Burgener, Jungi: "Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry." in: Veterinary immunology and immunopathology, Vol. 124, Issue 1-2, pp. 184-91, 2008 Tokumasa, Suto, Kagami, Furuta, Hirose, Watanabe, Saito, Shimoda, Iwamoto, Nakajima: "Expression of Tyk2 in dendritic cells is required for IL-12, IL-23, and IFN-gamma production and the induction of Th1 cell differentiation." in: Blood, Vol. 110, Issue 2, pp. 553-60, 2007 Pratesi, Petrangolini, Tortoreto, Addis, Belluco, Rossini, Selleri, Rumio, Menard, Balsari: "Therapeutic synergism of gemcitabine and CpG-oligodeoxynucleotides in an orthotopic human pancreatic carcinoma xenograft." in: Cancer research, Vol. 65, Issue 14, pp. 6388-93, 2005 Rutz, Metzger, Gellert, Luppa, Lipford, Wagner, Bauer: "Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pH-dependent manner." in: European journal of immunology, Vol. 34, Issue 9, pp. 2541-50, 2004 Rumio, Besusso, Palazzo, Selleri, Sfondrini, Dubini, Ménard, Balsari: "Degranulation of paneth cells via toll-like receptor 9." in: The American journal of pathology, Vol. 165, Issue 2, pp. 373-81, 2004 Ahmad-Nejad, Häcker, Rutz, Bauer, Vabulas, Wagner: "Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments." in: European journal of immunology, Vol. 32, Issue 7, pp. 1958-68, 2002 |
BilderProduktdetails anti-TLR9 Antikörper Target Details TLR9 Anwendungsinformationen Handhabung Referenzen für anti-TLR9 Antikörper (ABIN1106226) zurück nach oben |
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Bilder des Herstellers |