anti-Katze TLR3 Antikörper für Western Blotting

Recommended TLR3 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Toll-Like Receptor 3 (TLR3) Antikörper
  • CD283
  • IIAE2
  • AI957183
  • zgc:123006
  • toll like receptor 3
  • toll-like receptor 3
  • TLR3
  • Tlr3
  • tlr3
Epitop
C-Term, AA 800-904
32
26
15
7
7
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Reaktivität
Katze, Human, Schwein, Primate
283
215
63
46
6
5
3
3
2
2
2
2
2
2
1
1
1
1
Wirt
Kaninchen
199
122
43
3
3
Klonalität
Polyklonal
Konjugat
Dieser TLR3 Antikörper ist unkonjugiert
34
32
26
16
10
8
7
6
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
Western Blotting (WB)
237
148
104
90
68
45
43
34
13
12
11
7
5
4
1
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN410026
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN4360076 WB DyLight 405 Rabbit AA 800-904, C-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN4360075 WB FITC Rabbit AA 800-904, C-Term Anmelden zum Anzeigen Polyclonal 0

General

Antigen Toll-Like Receptor 3 (TLR3) Antikörper
Epitop C-Term, AA 800-904
(32), (26), (15), (7), (7), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reaktivität Katze, Human, Schwein, Primate
(283), (215), (63), (46), (6), (5), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Wirt Kaninchen
(199), (122), (43), (3), (3)
Klonalität
Polyklonal
Konjugat Dieser TLR3 Antikörper ist unkonjugiert
(34), (32), (26), (16), (10), (8), (7), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Western Blotting (WB)
(237), (148), (104), (90), (68), (45), (43), (34), (13), (12), (11), (7), (5), (4), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TLR3 Antikörper

Target Details TLR3 Anwendungsinformationen Handhabung Bilder
Reinigung Immunogen affinity purified
Immunogen Synthetic peptide made to a C-terminal portion of the human TLR3 protein (within residues 800-904). [Swiss-Prot# O15455]
Plasmids, Primers & others

Target Details TLR3

Produktdetails anti-TLR3 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung TLR3 (TLR3 Antibody Abstract)
Hintergrund Gene Symbol: TLR3
Molekulargewicht Theoretical MW: 105 kDa
Gen-ID 7098
UniProt O15455
Pathways TLR Signalweg, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-TLR3 Antikörper Target Details TLR3 Handhabung Bilder zurück nach oben
Applikations-hinweise Western Blot 1:500-1:1000This TLR3 antibody is useful for Western blot, where a band is seen at ~105 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for TLR3 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 25 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-TLR3 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TLR3 Antikörper Target Details TLR3 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.1 % Sodium Azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 4°C. Do not freeze.

Bilder

Produktdetails anti-TLR3 Antikörper Target Details TLR3 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Toll-Like Receptor 3 (TLR3) (AA 800-904), (C-Term) antibody (ABIN410026) Western Blot: TLR3 Antibody [ABIN4100261] - analysis of TLR3 in human macrophages usi...
Western Blotting (WB) image for anti-Toll-Like Receptor 3 (TLR3) (AA 800-904), (C-Term) antibody (ABIN410026) Western Blot: TLR3 Antibody [ABIN4100261] - Detection of TLR3 in Daudi whole cell lys...