TNFRSF12A Protein (Fc Tag,His tag)

Produktnummer ABIN7520592

Produktdetails

Target: TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses TNFRSF12A Protein ist gelabelt mit Fc Tag,His tag.

Verwendungszweck: Active Recombinant Human TNFRSF12A/TWEAKR/CD266 Protein

Sequenz: EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LW

Spezifität: Glu28-Trp79

Reinheit: > 90 % by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: 1.Measured by its ability to inhibit TWEAK-induced apoptosis in HT-29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 2-12 μg/mL in the presence of 1 μg/mL recombinant human TWEAK.|2.Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF12 at 2 μg/mL (100 μL/well) can bind Human TNFRSF12A with a linear range of 0.1-2.3 ng/mL.|3.Measured by its ability to inhibit the TWEAK-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 20-80 ng/mL in the presence of 15 ng/mL recombinant human TWEAK.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Antigendetails

Target: TNFRSF12A (Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A))

Andere Bezeichnung: TNFRSF12A/TWEAKR/CD266 (TNFRSF12A Produkte)

Synonyme: CD266 Protein, FN14 Protein, TWEAKR Protein, AI255180 Protein, C87282 Protein, Fn14 Protein, HPIP Protein, TWEAK-R Protein, TweakR Protein, TNF receptor superfamily member 12A Protein, tumor necrosis factor receptor superfamily, member 12a Protein, TNFRSF12A Protein, Tnfrsf12a Protein

Hintergrund:

Description: Fn14 (tumor necrosis factor receptor superfamily, member 12A), also known as TNFRSF12A, is the receptor for TNFSF12/TWEAK. Human and mouse TNFRSF12A share 82 % aa sequence identity. TNFRSF12A transcript was expressed at high levels in heart, placenta, and kidney, at intermediate levels in lung, skeletal muscle, and pancreas, and at low levels in brain and liver. In addition, elevated TNFRSF12A expression was found in human liver cancer cell lines and hepatocellular carcinoma specimens. TNFRSF12A is the weak inducer of apoptosis in some cell types. It promotes angiogenesis and the proliferation of endothelial cells. TNFRSF12A may modulate cellular adhesion to matrix proteins.

Name: CD266, FN14, TWEAKR,TNFRSF12A,FN14,TWEAKR

Gen-ID: 51330

UniProt: Q9NP84

Pathways: Apoptose, Regulation of Cell Size