EPO Protein (AVI tag,His tag)

Produktnummer ABIN7536398

Produktdetails

Target: EPO (Erythropoietin (EPO))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses EPO Protein ist gelabelt mit AVI tag,His tag.

Verwendungszweck: Recombinant Mouse Erythropoietin/EPO Protein

Sequenz: APPRLICDSR VLERYILEAK EAENVTMGCA EGPRLSENIT VPDTKVNFYA WKRMEVEEQA IEVWQGLSLL SEAILQAQAL LANSSQPPET LQLHIDKAIS GLRSLTSLLR VLGAQKELMS PPDTTPPAPL RTLTVDTFCK LFRVYANFLR GKLKLYTGEV CRRGDR

Spezifität: Ala27-Arg192

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: <0.001EU/μg

Biological Activity Comment: Recombinant Mouse EPO stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 for this effect is 4.3-17.3 ng/mL.corresponding to a specific activity of 5.78x104-2.33x105units/mg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Konzentration: 0.92 mg/mL

Buffer: Lyophilized from a 0.22 μm filtered solution of 20 mM NaAc, pH 4.5

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Antigendetails

Target: EPO (Erythropoietin (EPO))

Andere Bezeichnung: Erythropoietin/EPO (EPO Produkte)

Synonyme: EPO Protein, EP Protein, MVCD2 Protein, erythropoietin Protein, erythropoietin S homeolog Protein, erythropoietin a Protein, EPO Protein, epo Protein, epo.S Protein, Epo Protein, epoa Protein

Substanzklasse: Hormone

Hintergrund:

Description: Erythropoietin is a member of the EPO / TPO family. It is a secreted, glycosylated cytokine composed of four alpha helical bundles. Erythropoietin can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. Genetic variation in erythropoietin is associated with susceptbility to microvascular complications of diabetes type 2. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. It has a longer circulating half-life in vivo. Erythropoietin is being much misused as a performance-enhancing drug in endurance athletes.

Name: Erythropoietin,EPO

Gen-ID: 13856

UniProt: P07321

Pathways: JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity