EPO Protein (His tag)
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- Target Alle EPO Proteine anzeigen
- EPO (Erythropoietin (EPO))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
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Spezies
- Maus
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Quelle
- Human Cells
- Aufreinigungstag / Konjugat
- Dieses EPO Protein ist gelabelt mit His tag.
- Verwendungszweck
- Recombinant Mouse Erythropoietin/EPO (C-6His)
- Sequenz
- Ala27-Arg192
- Produktmerkmale
- Recombinant Mouse Erythropoietin is produced by our Mammalian expression system and the target gene encoding Ala27-Arg192 is expressed with a 6His tag at the C-terminus.
- Reinheit
- >95 % as determined by reducing SDS-PAGE.
- Endotoxin-Niveau
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.35 ng/mL.
- Top Product
- Discover our top product EPO Protein
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Erythropoietin (EPO) (AA 28-193) (Active) protein
EPO Spezies: Human Wirt: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Erythropoietin (EPO) (AA 1-193) protein (GST tag)EPO Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AA, AP
Erythropoietin (EPO) (AA 27-192) protein (His tag)Crystallography grade EPO Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- EPO (Erythropoietin (EPO))
- Andere Bezeichnung
- Erythropoietin/EPO (EPO Produkte)
- Synonyme
- EPO Protein, EP Protein, MVCD2 Protein, erythropoietin Protein, erythropoietin S homeolog Protein, erythropoietin a Protein, EPO Protein, epo Protein, epo.S Protein, Epo Protein, epoa Protein
- Substanzklasse
- Hormone
- Hintergrund
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Background: Erythropoietin (EPO) is a glycoprotein hormone that is principally known for its role in erythropoiesis, where it is responsible for stimulating proliferation and differentiation of erythroid progenitor cells. Erythropoietin is a member of the EPO/TPO family. It is a secreted, glycosylated cytokine composed of four alpha helical bundles. The differentiation of CFU-E (Colony Forming Unit-Erythroid) cells into erythrocytes can only be accomplished in the presence of EPO. Physiological levels of EPO in adult mammals are maintained primarily by the kidneys, whereas levels in fetal or neonatal mammals are maintained by the liver. EPO also can exert various non-hematopoietic activities, including vascularization and proliferation of smooth muscle, neural protection during hypoxia, and stimulation of certain B cells. Genetic variation in erythropoietin is associated with susceptbility to microvascular complications of diabetes type 2. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy.
Synonym: Erythropoietin, Epoetin, EPO
- Molekulargewicht
- 19.4 kDa
- UniProt
- Q0VED9
- Pathways
- JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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