BDNF Protein (His tag)

Produktnummer ABIN7535100

Produktdetails

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses BDNF Protein ist gelabelt mit His tag.

Verwendungszweck: Active Recombinant Human/Mouse/Rat BDNF Protein

Sequenz: HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRIDTSCV CTLTIKRGR

Spezifität: His129-Arg247

Reinheit: > 95 % by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: < 1.0 EU/μg of the protein by LAL method.

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human BDNF at 1 μg/mL (100 μL/well) can bind BDNF Rabbit mAb with a linear range of 0.4-2.15ng/mL.|2.Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human TrkB at 2 μg/mL (100 μL/well) can bind Recombinant Human BDNF with a linear range of 1.95-258 ng/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Antigendetails

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Andere Bezeichnung: BDNF (BDNF Produkte)

Synonyme: ANON2 Protein, BULN2 Protein, bdnf-A Protein, BDNF Protein, bdnf Protein, brain derived neurotrophic factor Protein, brain-derived neurotrophic factor Protein, brain-derived neurotrophic factor L homeolog Protein, brain-derived neurotrophic factor S homeolog Protein, BDNF Protein, Bdnf Protein, bdnf Protein, bdnf.L Protein, bdnf.S Protein

Hintergrund:

Description: Brain-derived neurotrophic factor (BDNF) is a member of the nerve growth factor family.The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins.BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor .

Name: ANON2, BULN2,BDNF,BULN2

Gen-ID: 627

UniProt: P23560-1

Pathways: RTK Signalweg, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity