Prothrombin Protein (AA 25-617) (His tag)

Produktnummer ABIN7504306

Produktdetails

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 25-617

Spezies: Ratte

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Prothrombin Protein ist gelabelt mit His tag.

Verwendungszweck: Rat Coagulation Factor II Protein

Sequenz: Gln25-Arg617

Produktmerkmale: Recombinant Rat Coagulation Factor II Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln25-Arg617.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Andere Bezeichnung: Coagulation Factor II (F2 Produkte)

Synonyme: PT Protein, RPRGL2 Protein, THPH1 Protein, Cf-2 Protein, Cf2 Protein, FII Protein, thrombin Protein, zgc:66319 Protein, wu:fb57c10 Protein, wu:fd42e09 Protein, wu:fd59d01 Protein, prothrombin Protein, F2 Protein, coagulation factor II, thrombin Protein, coagulation factor II Protein, coagulation factor II (thrombin) Protein, F2 Protein, f2 Protein

Hintergrund: Prothrombin, or coagulation factor II, is a multidomain zymogen precursor of thrombin that undergoes an allosteric equilibrium between two alternative conformations, open and closed, that react differently with the physiological activator prothrombinase. It has 10 sites of gamma-carboxylation, which are required for its bioactivity, and is N-glycosylated at three of four putative sites.

Molekulargewicht: 69.12 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

NCBI Accession: NP_075213

Pathways: Komplementsystem, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling