TNFRSF10B Protein (AA 56-182) (His-Avi Tag)

Produktnummer ABIN7275779

Produktdetails

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Protein-Typ: Recombinant

Proteineigenschaft: AA 56-182

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses TNFRSF10B Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: Human TRAIL R2/DR5/TNFRSF10B Protein

Sequenz: Ile56-Glu182

Produktmerkmale: Recombinant Human TRAIL R2/DR5/TNFRSF10B Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile56-Glu182.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TRAIL R2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TRAIL R2 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Andere Bezeichnung: TRAIL R2 (TNFRSF10B Produkte)

Synonyme: TNFRSF10B Protein, dr5 Protein, cd262 Protein, killer Protein, trick2 Protein, trickb Protein, xDR-M1 Protein, xDR-M2 Protein, ztnfr9 Protein, trailr2 Protein, trick2a Protein, trick2b Protein, CD262 Protein, DR5 Protein, KILLER Protein, KILLER/DR5 Protein, TRAIL-R2 Protein, TRAILR2 Protein, TRICK2 Protein, TRICK2A Protein, TRICK2B Protein, TRICKB Protein, ZTNFR9 Protein, Ly98 Protein, MK Protein, tumor necrosis factor receptor superfamily, member 10b Protein, tumor necrosis factor receptor superfamily member 10b S homeolog Protein, tumor necrosis factor receptor superfamily member 10b L homeolog Protein, tumor necrosis factor receptor superfamily member 10b Protein, TNF receptor superfamily member 10b Protein, Tnfrsf10b Protein, TNFRSF10B Protein, tnfrsf10b.S Protein, tnfrsf10b.L Protein, tnfrsf10b Protein

Hintergrund: DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.

Molekulargewicht: 17.2 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.

Pathways: p53 Signalweg, Apoptose, Positive Regulation of Endopeptidase Activity