Osteopontin Protein (AA 17-294) (His tag)

Produktnummer ABIN7275648

Produktdetails

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 17-294

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Osteopontin Protein ist gelabelt mit His tag.

Verwendungszweck: Mouse SPP1/OPN Protein

Sequenz: Leu17-Asn294

Produktmerkmale: Recombinant Mouse SPP1/OPN Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu17-Asn294.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Andere Bezeichnung: SPP1 (SPP1 Produkte)

Synonyme: BNSP Protein, BSPI Protein, ETA-1 Protein, OPN Protein, 2AR Protein, Apl-1 Protein, Bsp Protein, Eta Protein, OP Protein, Opn Protein, Opnl Protein, Ric Protein, Spp-1 Protein, OSP Protein, zgc:111821 Protein, secreted phosphoprotein 1 Protein, SPP1 Protein, Spp1 Protein, spp1 Protein

Hintergrund: Secreted phosphoprotein 1 (SPP1) expression in TAMs isolated from lung adenocarcinoma tissues and PMA-treated THP-1 cells were measured. Macrophage polarization was identified by flow cytometric analysis. Cell migration and apoptosis were assessed by Transwell migration assays and flow cytometric analysis, respectively. SPP1 is highly expressed in tumor tissues and TAMs isolated from patients with an advanced TNM stage, and also in PMA-treated THP-1 cells.

Molekulargewicht: 31.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

UniProt: P10923

Pathways: Regulation of Cell Size