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Osteopontin Protein (AA 17-287) (His tag)

SPP1 Spezies: Human Wirt: Escherichia coli (E. coli) > 95 % AcA, CC Active
Produktnummer ABIN6239742
  • Target Alle Osteopontin (SPP1) Proteine anzeigen
    Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 17-287
    Spezies
    • 25
    • 7
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 21
    • 15
    • 8
    • 3
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses Osteopontin Protein ist gelabelt mit His tag.
    Applikation
    Activity Assay (AcA), Cell Culture (CC)
    Produktmerkmale
    Tag location: N-terminal His Tag
    Reinheit
    > 95 %
    Biological Activity Comment
    Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which would promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1x105 cells/mL, and treated with or without OPN(100ng/ml, 200ng/ml) for 24h and MCP-1 levels in the cell supernatant were determined by ELISA. MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN, the data was shown in Table 1 and Figure 1. Sample O.D. value Corrected Concentration of MCP-1(cell supernatant of MCF-7 cells) (ng/mL) stimulated with OPN (100ng/mL) 0.970 0.922 1.72 stimulated with OPN (200ng/mL) 1.155 1.107 2.11 unstimulated 0.674 0.626 1.11 Table 1. MCP-1 levels in the cell supernatant of MCF-7 cells regulated by OPN Figure 1. MCP-1 levels in the cell supernatant of MCF-7 cells regulated by OPN.
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  • Applikationshinweise
    Isoelectric Point: 4.3
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Konservierungsmittel
    Dithiothreitol (DTT), Other preservative, ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
    Andere Bezeichnung
    Osteopontin (OPN) (SPP1 Produkte)
    Synonyme
    BNSP Protein, BSPI Protein, ETA-1 Protein, OPN Protein, 2AR Protein, Apl-1 Protein, Bsp Protein, Eta Protein, OP Protein, Opn Protein, Opnl Protein, Ric Protein, Spp-1 Protein, OSP Protein, zgc:111821 Protein, secreted phosphoprotein 1 Protein, SPP1 Protein, Spp1 Protein, spp1 Protein
    Hintergrund
    Alternative Names: SPP1, BNSP, BSPI, ETA1, Secreted Phosphoprotein 1, Bone Sialoprotein I, Early T-Lymphocyte Activation 1, Nephropontin, Urinary stone protein, Uropontin
    Molekulargewicht
    60kDa
    UniProt
    P10451
    Pathways
    Regulation of Cell Size
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