Osteopontin Protein (AA 17-317) (His tag)
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- Target Alle Osteopontin (SPP1) Proteine anzeigen
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 17-317
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Spezies
- Ratte
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses Osteopontin Protein ist gelabelt mit His tag.
- Applikation
- Activity Assay (AcA), Cell Culture (CC)
- Produktmerkmale
- Tag location: N-terminal His Tag
- Reinheit
- > 95 %
- Biological Activity Comment
- Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1x105 cells/mL, and treated with or without various concentrations of OPN for 24h and MCP-1 levels in the cell supernatant were determined by ELISA. MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN, the data was shown in Table 1 and Figure 1. Sample Concentration of MCP-1 O.D. value Corrected (cell supernatant of MCF-7 cells) (ng/mL) stimulated with OPN (100ng/mL) 1.358 1.305 2.53 stimulated with OPN (200ng/mL) 1.177 1.124 2.15 stimulated with OPN (400ng/mL) 1.101 1.048 1.99 unstimulated 0.944 0.891 1.66 Table 1. MCP-1 levels in the cell supernatant of MCF-7 cells regulated by OPN MCP-1 levels in the cell supernatant of MCF-7 cells regulated by OPN.
- Top Product
- Discover our top product SPP1 Protein
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- Applikationshinweise
- Isoelectric Point: 4.3
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Konservierungsmittel
- Dithiothreitol (DTT), Other preservative, ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- Andere Bezeichnung
- Osteopontin (OPN) (SPP1 Produkte)
- Synonyme
- BNSP Protein, BSPI Protein, ETA-1 Protein, OPN Protein, 2AR Protein, Apl-1 Protein, Bsp Protein, Eta Protein, OP Protein, Opn Protein, Opnl Protein, Ric Protein, Spp-1 Protein, OSP Protein, zgc:111821 Protein, secreted phosphoprotein 1 Protein, SPP1 Protein, Spp1 Protein, spp1 Protein
- Hintergrund
- Alternative Names: SPP1, BNSP, BSPI, ETA1, Secreted Phosphoprotein 1, Bone Sialoprotein I, Early T-Lymphocyte Activation 1, Nephropontin, Urinary stone protein, Uropontin
- Molekulargewicht
- 60kDa
- UniProt
- P10451
- Pathways
- Regulation of Cell Size
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