IL-13 Protein (AA 21-132) (His-Avi Tag)

Produktnummer ABIN7274900

Produktdetails

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Protein-Typ: Recombinant

Proteineigenschaft: AA 21-132

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses IL-13 Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: Human IL-13 Protein

Sequenz: Gly21-Asn132

Produktmerkmale: Recombinant Human IL-13 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gly21-Asn132.

Reinheit: > 90 % as determined by Tris-Bis PAGE

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-13, His Tag at 1μg/ml (100μl/Well). Dose response curve for Human IL-13Ra1, hFc Tag with the EC50 of 2.7μg/ml determined by ELISA. The affinity constant of 0.7μM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Andere Bezeichnung: IL-13 (IL13 Produkte)

Synonyme: IL13 Protein, IL-13 Protein, P600 Protein, Il-13 Protein, L13 Protein, interleukin 13 Protein, IL13 Protein, Il13 Protein

Hintergrund: Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).

Molekulargewicht: 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 25-45 kDa based on Tris-Bis PAGE result.

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport