HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
Protein-Typ
Recombinant
Proteineigenschaft
AA 32-728
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses HGF Protein ist gelabelt mit Fc Tag.
Verwendungszweck
Human HGF Protein
Sequenz
Gln32-Ser728
Produktmerkmale
Recombinant Human HGF Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln32-Ser728.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human HGF R, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
HGF
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS, 100 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types, plays a key role in β-cell homeostasis, and is capable of modulating the inflammatory response.HGF plays a central role in these metabolic disorders,HGF levels could be employed as a biomarker for disease status/progression, and HGF/c-Met signaling pathway modulators could effectively regulate IR and treat diabetes.
Molekulargewicht
the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.