Dieses Glypican 3 Protein ist gelabelt mit His tag.
Verwendungszweck
Cynomolgus GPC3/Glypican 3 Protein
Sequenz
Gln25-His559
Produktmerkmale
Recombinant Cynomolgus GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln25-His559.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus GPC3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA. See testing image for detail.
GPC3
Spezies: Human
Wirt: Wheat germ
Recombinant
WB, ELISA, AA, AP
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
Molekulargewicht
61.8 kDa. Due to glycosylation, the protein migrates to 42 kDa, 70-135 kDa based on Tris-Bis PAGE result.