Glypican 3 Protein (GPC3) (AA 25-559) (His tag,AVI tag,Biotin)

Produktnummer ABIN5954920

Produktdetails

Target: Glypican 3 (GPC3)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 25-559

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Glypican 3 Protein ist gelabelt mit His tag,AVI tag,Biotin.

Sequenz: AA 25-559

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Reinheit: >85 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen

Berman, Kelada, Gutsche, Natarajan, Swenson, Fu, Hong, Ho, Choyke, Escorcia: "In Vitro Performance of Published Glypican 3-Targeting Peptides TJ12P1 and L5 Indicates Lack of Specificity and Potency." in: Cancer biotherapy & radiopharmaceuticals, (2019) (PubMed).

Antigendetails

Target: Glypican 3 (GPC3)

Andere Bezeichnung: Glypican 3 (GPC3 Produkte)

Synonyme: GPC3 Protein, sgb Protein, dgsx Protein, sdys Protein, sgbs Protein, oci-5 Protein, sgbs1 Protein, DGSX Protein, GTR2-2 Protein, MXR7 Protein, OCI-5 Protein, SDYS Protein, SGB Protein, SGBS Protein, SGBS1 Protein, Glypican-3 Protein, glypican 3 Protein, gpc3 Protein, GPC3 Protein, Gpc3 Protein

Hintergrund: Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Molekulargewicht: 38.1 kDa and 26.4 kDa

NCBI Accession: NP_004475

Pathways: Glycosaminoglycan Metabolic Process