SARS-CoV-2 Spike S1 Protein (D614G) (His-Avi Tag)
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- Target Alle SARS-CoV-2 Spike S1 Proteine anzeigen
- SARS-CoV-2 Spike S1
- Protein-Typ
- Recombinant
- Proteineigenschaft
- D614G
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Spezies
- SARS Coronavirus-2 (SARS-CoV-2)
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses SARS-CoV-2 Spike S1 Protein ist gelabelt mit His-Avi Tag.
- Verwendungszweck
- SARS-COV-2 Spike S1 (D614G) Protein
- Sequenz
- Gln14-Arg683 (D614G)
- Produktmerkmale
- Recombinant SARS-COV-2 Spike S1 (D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln14-Arg683(D614G).
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2μg/ml (100μl/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 42ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product SARS-CoV-2 Spike S1 Protein
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- SARS-CoV-2 Spike S1
- Abstract
- SARS-CoV-2 Spike S1 Produkte
- Synonyme
- E2 Protein, Surface Glycoprotein Protein, S Protein
- Substanzklasse
- Viral Protein
- Hintergrund
- The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.
- Molekulargewicht
- 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.
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