CLEC5A Protein (AA 26-188) (Fc Tag)

Produktnummer ABIN7274355

Produktdetails

Target: CLEC5A (C-Type Lectin Domain Family 5, Member A (CLEC5A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 26-188

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CLEC5A Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Human MDL-1/CLEC5A Protein

Sequenz: Tyr26-Lys188

Produktmerkmale: Recombinant Human MDL-1/CLEC5A Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Tyr26-Lys188.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human MDL-1, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-MDL-1 Antibody, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: CLEC5A (C-Type Lectin Domain Family 5, Member A (CLEC5A))

Andere Bezeichnung: MDL-1 (CLEC5A Produkte)

Synonyme: CLECSF5 Protein, MDL-1 Protein, MDL1 Protein, Clecsf5 Protein, Ly100 Protein, Mdl1 Protein, C-type lectin domain containing 5A Protein, C-type lectin domain family 5, member a Protein, CLEC5A Protein, Clec5a Protein

Hintergrund: In C. elegans, increased lifespan in daf-2 insulin/IGF-1 receptor mutants is accompanied by up-regulation of the MDL-1 Mad basic helix-loop-helix leucine zipper transcription factor.MDL-1, like its mammalian orthologs, is an inhibitor of cell proliferation and growth that slows progression of an age-related pathology in C. elegans (uterine tumors). In addition, intestine-limited rescue of mdl-1 increased lifespan but not to wild type levels. Thus, mdl-1 likely acts both in the intestine and the germline to influence age-related mortality.

Molekulargewicht: 46 kDa. Due to glycosylation, the protein migrates to 63-70 kDa based on Tris-Bis PAGE result.