CD25 Protein (AA 22-213) (His-Avi Tag)
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- Target Alle CD25 (IL2RA) Proteine anzeigen
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 22-213
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses CD25 Protein ist gelabelt mit His-Avi Tag.
- Verwendungszweck
- Human IL-2 R alpha/CD25 Protein
- Sequenz
- Glu22-Cys213
- Produktmerkmale
- Recombinant Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu22-Cys213.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-2 R alpha, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA. The affinity constant of 15.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product IL2RA Protein
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- Andere Bezeichnung
- IL-2 R alpha (IL2RA Produkte)
- Synonyme
- CD25 Protein, IDDM10 Protein, IL2R Protein, TCGFR Protein, IL2RAC Protein, IL-2RA Protein, Il2r Protein, Ly-43 Protein, interleukin 2 receptor subunit alpha Protein, interleukin-2 receptor subunit alpha-like Protein, interleukin 2 receptor, alpha chain Protein, IL2RA Protein, Il2ra Protein, LOC100222957 Protein
- Hintergrund
- Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL-2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
- Molekulargewicht
- 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
- UniProt
- P01589
- Pathways
- JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation
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