TNFRSF13C
(Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))
Protein-Typ
Recombinant
Proteineigenschaft
AA 7-71
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses TNFRSF13C Protein ist gelabelt mit Fc Tag.
Verwendungszweck
Human BAFFR/TNFRSF13C Protein
Sequenz
Ser7-Ala71
Produktmerkmale
Recombinant Human BAFFR/TNFRSF13C Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser7-Ala71.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human BAFF Trimer, His Tag at 0.5μg/ml (100μl/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 0.73μg/ml determined by ELISA. See testing image for detail.
TNFRSF13C
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
ELISA
TNFRSF13C
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
TNFRSF13C
(Tumor Necrosis Factor Receptor Superfamily, Member 13C (TNFRSF13C))
BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF.
Molekulargewicht
33.3 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Tris-Bis PAGE result.